Zhang Ying-Ying, Han Xue, Liu Ye, Chen Jian, Hua Lei, Ma Qian, Huang Yang-Yu-Xin, Tang Qiong-Yao, Zhang Zhe
School of Anesthesiology, Xuzhou Medical University, Xuzhou, Jiangsu Province, China.
Jiangsu Province Key Laboratory of Anesthesiology, Xuzhou Medical University, Xuzhou, Jiangsu Province, China.
PLoS One. 2018 Jan 25;13(1):e0191749. doi: 10.1371/journal.pone.0191749. eCollection 2018.
LRRC55 (leucine-rich repeat-containing protein 55) protein is an auxiliary γ subunit of BK (Big conductance potassium channel) channels, which leftward shifts GVs of BK channels around 50 mV in the absence of cytosolic Ca2+. LRRC55 protein is also the only γ subunit of BK channels that is expressed in mammalian nervous system. However, the expression pattern of LRRC55 gene in adult mammalian brain remains elusive. In this study, we investigated the distribution of LRRC55 mRNA in the adult mouse brain by using in situ hybridization. We found that LRRC55 mRNA is richly expressed in the adult mouse medial habenula nucleus (MHb), cerebellum and pons. However, the potential role of LRRC55 in MHb and cerebellum could be different based on the function of BK channels in these brain regions.
富含亮氨酸重复序列蛋白55（LRRC55）是大电导钾通道（BK通道）的辅助γ亚基，在没有胞质Ca2+的情况下，它会使BK通道的电压依赖性激活向左移动约50 mV。LRRC55蛋白也是BK通道在哺乳动物神经系统中表达的唯一γ亚基。然而，LRRC55基因在成年哺乳动物大脑中的表达模式仍不清楚。在本研究中，我们通过原位杂交研究了LRRC55 mRNA在成年小鼠大脑中的分布。我们发现LRRC55 mRNA在成年小鼠内侧缰核（MHb）、小脑和脑桥中大量表达。然而，基于BK通道在这些脑区的功能，LRRC55在MHb和小脑中的潜在作用可能不同。
