Shi Xiu-Zhen, Feng Xiao-Wu, Sun Jie-Jie, Zhao Xiao-Fan, Wang Jin-Xing
Shandong Provincial Key Laboratory of Animal Cells and Developmental Biology, School of Life Sciences, Shandong University, Jinan, Shandong, 250100, China.
Shandong Provincial Key Laboratory of Animal Cells and Developmental Biology, School of Life Sciences, Shandong University, Jinan, Shandong, 250100, China.
Fish Shellfish Immunol. 2017 Feb;61:130-137. doi: 10.1016/j.fsi.2016.12.029. Epub 2016 Dec 25.
Leucine rich repeat (LRR) motif exists in many immune receptors of animals and plants. Most LRR containing (LRRC) proteins are involved in protein-ligand and protein-protein interaction, but the exact functions of most LRRC proteins were not well-studied. In this study, an LRRC protein was identified from kuruma shrimp Marsupenaeus japonicus, and named as MjLRRC1. MjLRRC1 was consistently expressed in different tissues of normal shrimp with higher expression in gills and stomach. At the transcriptional level, there were no significant changes of MjLRRC1 after injection of Vibrio anguillarum or Staphylococcus aureus in gills and hepatopancreas. While in V. anguillarum oral infection, MjLRRC1 was upregulated in stomach but not in intestine. The recombinant MjLRRC1 protein could bind to Gram-positive and Gram-negative bacteria, bacterial cell wall components including peptidoglycan, lipoteichoic acid, and lipopolysaccharide. MjLRRC1 regulated the expression of some antimicrobial peptide (AMP) genes and participated in bacteria clearance of stomach. All these results suggested that MjLRRC1 might play important roles in antibacterial immune response of kuruma shrimp.
富含亮氨酸重复序列（LRR）基序存在于动植物的许多免疫受体中。大多数含LRR的（LRRC）蛋白参与蛋白质-配体和蛋白质-蛋白质相互作用，但大多数LRRC蛋白的确切功能尚未得到充分研究。在本研究中，从日本对虾中鉴定出一种LRRC蛋白，并将其命名为MjLRRC1。MjLRRC1在正常对虾的不同组织中持续表达，在鳃和胃中表达较高。在转录水平上，在鳃和肝胰腺中注射鳗弧菌或金黄色葡萄球菌后，MjLRRC1没有显著变化。而在鳗弧菌口服感染时，MjLRRC1在胃中上调，但在肠道中没有上调。重组MjLRRC1蛋白可以结合革兰氏阳性菌和革兰氏阴性菌、细菌细胞壁成分，包括肽聚糖、脂磷壁酸和脂多糖。MjLRRC1调节一些抗菌肽（AMP）基因的表达，并参与胃中的细菌清除。所有这些结果表明，MjLRRC1可能在日本对虾的抗菌免疫反应中发挥重要作用。
