Department of Agronomy, Zhejiang Key Laboratory of Crop Germplasm, Zhejiang University, Hangzhou, 310058, China.
School of Biological Science and Technology, University of Jinan, Jinan, 250022, China.
BMC Genomics. 2018 Jan 25;19(1):81. doi: 10.1186/s12864-018-4483-6.
The interest has been increasing on the phenolic compounds in plants because of their nutritive function as food and the roles regulating plant growth. However, their underlying genetic mechanism in barley is still not clear.
A genome-wide association study (GWAS) was conducted for total phenolic content (TPC), total flavonoid content (FLC) and antioxidant activity (AOA) in 67 cultivated and 156 Tibetan wild barley genotypes. Most markers associated with phenolic content were different in cultivated and wild barleys. The markers bPb-0572 and bPb-4531 were identified as the major QTLs controlling phenolic compounds in Tibetan wild barley. Moreover, the marker bPb-4531 was co-located with the UDP- glycosyltransferase gene (HvUGT), which is a homolog to Arabidopsis UGTs and involved in biosynthesis of flavonoid glycosides .
GWAS is an efficient tool for exploring the genetic architecture of phenolic compounds in the cultivated and Tibetan wild barleys. The DArT markers applied in this study can be used in barley breeding for developing new barley cultivars with higher phenolics content. The candidate gene (HvUGT) provides a potential route for deep understanding of the molecular mechanism of flavonoid synthesis.
由于植物中的酚类化合物具有作为食物的营养功能和调节植物生长的作用，因此人们对其越来越感兴趣。然而，它们在大麦中的潜在遗传机制尚不清楚。
对 67 个栽培品种和 156 个西藏野生大麦基因型的总酚含量（TPC）、总黄酮含量（FLC）和抗氧化活性（AOA）进行了全基因组关联研究（GWAS）。与酚含量相关的大多数标记在栽培大麦和野生大麦中是不同的。标记 bPb-0572 和 bPb-4531 被鉴定为控制西藏野生大麦中酚类化合物的主要 QTL。此外，标记 bPb-4531 与 UDP-糖基转移酶基因（HvUGT）共定位，HvUGT 是拟南芥 UGTs 的同源物，参与类黄酮糖苷的生物合成。
GWAS 是研究栽培大麦和西藏野生大麦中酚类化合物遗传结构的有效工具。本研究中应用的 DArT 标记可用于大麦育种，以开发具有更高酚含量的新型大麦品种。候选基因（HvUGT）为深入了解类黄酮合成的分子机制提供了一条潜在途径。
