Department of clinical laboratory, The first Affiliated Hospital of Nanchang university, Nanchang, 330006, China.
Department of emergency, The first Affiliated Hospital of Nanchang University, Nanchang, 330006, China.
BMC Infect Dis. 2018 Jan 25;18(1):51. doi: 10.1186/s12879-018-2955-6.
S.aureus is a predominant pathogen that causes infection in critically ill patients, but little information exists regarding the characterization of S. aureus from different sources in burn patients in southeastern China.
We enrolled 125 patients with S. aureus infection in burns center between Jan 2014 and Dec 2015. S. aureus isolates were characterized by antimicrobial susceptibility test, toxin gene detection, and molecular typing with multilocus sequence type, staphylococcal protein A (spa) type, and staphylococcal cassette chromosome mec (SCCmec) type.
Sixty-eight MRSA were isolated from SSTI and 31 from non-SSTI patients, respectively. Overall, the drug-resistant ability of S. aureus isolated from SSTI was higher than that from non-SSTI groups. SCCmecIII-CC239-t030 was the most common clone (38 from SSTIs, and 8 from non-SSTIs). Seg was the most common enterotoxin gene (21 from SSTIs and 33 from non-SSTIs). Isolates from SSTIs was more likely to carry seb (P = 0.04), while those from non-SSTIs tended to carry sea and seg (P = 0.002 and 0.01, respectively). Although isolates carried four hemolysin genes, there was no significant difference between them (P > 0.05).
SCCmecIII-CC239-t030 was the most common clone in Jiangxi burns center, China. The molecular characterization of S. aureus was quite different between SSTI and non-SSTI groups.
金黄色葡萄球菌是引起危重症患者感染的主要病原体，但有关中国东南部烧伤患者不同来源金黄色葡萄球菌的特征描述信息较少。
我们纳入了 2014 年 1 月至 2015 年 12 月在烧伤中心的 125 例金黄色葡萄球菌感染患者。采用药敏试验、毒素基因检测和多位点序列分型（MLST）、葡萄球菌蛋白 A（spa）型和葡萄球菌盒式染色体 mec（SCCmec）型对金黄色葡萄球菌分离株进行特征分析。
分别从 SSTI 和非 SSTI 患者中分离出 68 株耐甲氧西林金黄色葡萄球菌（MRSA）和 31 株。总体而言，SSTI 中分离出的金黄色葡萄球菌耐药能力高于非 SSTI 组。SCCmecIII-CC239-t030 是最常见的克隆（38 株来自 SSTI，8 株来自非 SSTI）。Seg 是最常见的肠毒素基因（21 株来自 SSTI，33 株来自非 SSTI）。SSTI 分离株更容易携带 seb（P=0.04），而非 SSTI 分离株则更倾向于携带 sea 和 seg（P=0.002 和 0.01）。尽管分离株携带四个溶血素基因，但无明显差异（P>0.05）。
SCCmecIII-CC239-t030 是中国江西烧伤中心最常见的克隆。SSTI 和非 SSTI 组之间金黄色葡萄球菌的分子特征差异较大。
