Real-Time Approach to Flow Cell Imaging of Candida albicans Biofilm Development.

The ability of to form biofilms is a virulence factor that allows tissue attachment and subsequent infection of host tissues. Fungal biofilms have been particularly well studied, however the vast majority of these studies have been conducted under static conditions. Oral biofilms form in the presence of salivary flow, therefore we developed a novel flow system used for real-time imaging of fungal biofilm development. wild-type (WT) cells readily attached to the substrate surface during the 2 h attachment phase, then formed heterogeneous biofilms after 18 h flow. Quantitative values for biomass, rates of attachment and detachment, and cell-cell adhesion events were obtained for WT cells and for a hyperfilamentous mutant Δ. Attachment rates of WT cells were nearly 2-fold higher than Δ cells, although Δ cells formed 4-fold higher biomass. The reduced normalized detachment rate was the primary factor responsible for the increased biomass of Δ biofilm, showing that cell detachment rates are an important predictor for ultimate biofilm mass under flow. Unlike static biofilms, cells under constant laminar flow undergo continuous detachment and seeding that may be more representative of the development of in vivo biofilms.