Shepard A D, Eldrup-Jorgensen J, Keough E M, Foxall T F, Ramberg K, Connolly R J, Mackey W C, Gavris V, Auger K R, Libby P
Surgery. 1986 Mar;99(3):318-26.
Endothelial cell (EC) seeding has been proposed as a method to improve the performance of small-caliber synthetic vascular prostheses. Seeding experiments to date have all been carried out in the dog. This study investigates EC seeding of small-caliber Dacron carotid interposition grafts compared with contralateral control grafts in the baboon. Surface thrombogenicity was assessed at 24 hours, 2 weeks, and 4 weeks after implantation with indium 111-labeled autologous platelets. Morphologic and immunohistochemical techniques were used to assess the identity and homogeneity of the EC inoculum before seeding and to identify cell types on the harvested grafts. There was no significant difference in patency rates between seeded and control grafts at 5 weeks. Platelet accumulation on seeded grafts was significantly less (p less than 0.05) than on paired controls at 2 and 4 weeks after implantation. The luminal lining of seeded grafts had more cellular ingrowth, less adherent thrombus, and more surface cells with the morphologic and histochemical characteristics of EC than did the lining of controls. EC seeding reduces the platelet reactivity and accelerates EC coverage of small-caliber grafts in the baboon.
内皮细胞（EC）接种已被提议作为一种改善小口径合成血管移植物性能的方法。迄今为止，接种实验均在狗身上进行。本研究调查了在狒狒身上，小口径涤纶颈动脉置换移植物的内皮细胞接种与对侧对照移植物的情况。在植入后24小时、2周和4周，用铟111标记的自体血小板评估表面血栓形成性。形态学和免疫组织化学技术用于评估接种前内皮细胞接种物的一致性和同质性，并识别收获移植物上的细胞类型。在5周时，接种移植物和对照移植物的通畅率没有显著差异。在植入后2周和4周，接种移植物上的血小板积聚明显少于配对对照（p小于0.05）。与对照移植物的内膜相比，接种移植物的管腔内膜有更多的细胞向内生长、更少的附着血栓以及更多具有内皮细胞形态和组织化学特征的表面细胞。内皮细胞接种可降低狒狒小口径移植物的血小板反应性并加速内皮细胞覆盖。
