Jensen N, Lindblad B, Ljungberg J, Leide S, Bergqvist D
Department of Experimental Research and Surgery, University Hospital, Malmö, Sweden.
Ann Vasc Surg. 1996 Nov;10(6):530-6. doi: 10.1007/BF02000441.
Endothelial cell seeding has been advocated as a method for reducing the thrombogenicity of prosthetic grafts. Principally two different techniques for endothelial cell seeding can be used: immediate seeding of grafts followed by implantation or initial growth and establishment of an endothelial cell-covered surface before subsequent late implantation. This study was designed to determine whether the immediate seeding technique altered thrombogenicity directly after graft implantation. Carotid arteries from 19 sheep were replaced with Dacron interposition grafts; one side was seeded with endothelial cells and the other side was left unseeded. The dynamics of thrombus formation involving radiolabeled platelets, leukocytes, and fibrinogen were studied for 4 hours with flow reduced to 35 ml/min. No difference in platelet uptake (approximately 6-fold increase compared to baseline values) was found between endothelial cell seeded and unseeded grafts. Likewise, there were no differences in leukocyte uptake (approximately 4-fold increase) or fibrinogen uptake (approximately 10- to 15-fold increase) between the two groups. No differences were demonstrated with regard to patency or thrombus weight. In this experimental investigation we were unable to verify any change in the uptake of platelets, white blood cells, or fibrinogen between endothelial cell seeded and unseeded Dacron grafts during the first 4 hours after graft placement. Immediate seeding does not affect the initial thrombogenicity of grafts.
内皮细胞接种已被提倡作为一种降低人工血管血栓形成倾向的方法。主要有两种不同的内皮细胞接种技术：血管移植后立即接种然后植入，或在随后的晚期植入前先进行内皮细胞的初始生长并形成覆盖内皮细胞的表面。本研究旨在确定立即接种技术在血管移植后是否直接改变血栓形成倾向。用涤纶血管置换19只绵羊的颈动脉；一侧接种内皮细胞，另一侧不接种。在血流速度降至35 ml/min的情况下，对涉及放射性标记血小板、白细胞和纤维蛋白原的血栓形成动态进行了4小时的研究。在内皮细胞接种血管和未接种血管之间，未发现血小板摄取量有差异（与基线值相比增加约6倍）。同样，两组之间白细胞摄取量（增加约4倍）或纤维蛋白原摄取量（增加约10至15倍）也没有差异。在通畅性或血栓重量方面未显示出差异。在本实验研究中，我们无法证实血管移植后最初4小时内，接种内皮细胞的涤纶血管和未接种血管在血小板、白细胞或纤维蛋白原摄取方面有任何变化。立即接种不影响血管移植后的初始血栓形成倾向。
