Division of Engineering, New York University Abu Dhabi, United Arab Emirates.
Lab Chip. 2018 Feb 13;18(4):679-687. doi: 10.1039/c7lc00998d.
In this study, we report the use of a high-throughput microfluidic spiral chip to screen out eggs from a mixed age nematode population, which can subsequently be cultured to a desired developmental stage. For the sorting of a mixture containing three different developmental stages, eggs, L1 and L4, we utilized a microfluidic spiral chip with a trapezoidal channel to obtain a sorting efficiency of above 97% and a sample purity (SP) of above 80% for eggs at different flow rates up to 10 mL min. The result demonstrated a cost-effective, simple, and highly efficient method for synchronizing C. elegans at a high throughput (∼4200 organisms per min at 6 mL min), while eliminating challenges such as clogging and non-reusability of membrane-based filtration. Due to its simplicity, our method can be easily adopted in the C. elegans research community.
在这项研究中，我们报告了使用高通量微流控螺旋芯片从混合年龄线虫群体中筛选出卵，随后可以将其培养到所需的发育阶段。对于含有三个不同发育阶段（卵、L1 和 L4）的混合物进行分选，我们利用具有梯形通道的微流控螺旋芯片，在高达 10 mL min 的不同流速下，获得了高于 97%的分选效率和高于 80%的卵样品纯度（SP）。该结果展示了一种经济高效、简单且高效的方法，可用于以高通量（在 6 mL min 时约为 4200 个生物体/分钟）同步秀丽隐杆线虫，同时消除了基于膜过滤的堵塞和不可重复使用等挑战。由于其简单性，我们的方法可以很容易地被秀丽隐杆线虫研究界采用。
