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基于Rc和Pb基因分型的有色稻壳水稻的预育种选择

[Prebreeding selection of rice with colored pericarp based on genotyping Rc and Pb genes].

作者信息

Rysbekova A B, Kazkeyev D T, Usenbekov B N, Mukhina Zh M, Zhanbyrbaev E A, Sartbaeva I A, Zhambakin K Zh, Berkimbay Kh A, Batayeva D S

出版信息

Genetika. 2017 Jan;53(1):43-53.

Abstract

The research was aimed at developing prebreeding resources of Kazakhstan rice varieties with colored pericarp for breeding. During the study, hybrid analysis of inheritance of the trait “colored pericarp” in breeding material used for the work was performed. Rice genotypes with colored pericarp, as well as white rice varieties possessing important breeding traits and maturing under conditions of the republic, were selected from the collection of the Institute of Plant Biology and Biotechnology, Republic of Kazakhstan. Identification of allelic status of Rc (red pericarp) and Pb (anthocyanin pericarp) genes was performed for selected samples using the PCR method. When selecting parental forms for crossing, foreign rice varieties with colored pericarp (Rubin, Mavr, Black rice, etc.) were used as recipient forms. As donors, we used local white rice varieties of Kazakhstan breeding adapted to the soil and climate conditions of rice growing regions (Madina, Marzhan, Bakanasskiy, PakLi) as well as foreign varieties. The ability to set hybrid caryopses and the percentage of sterility were determined in obtained F1 rice hybrids. As a result, the most promising prebreeding material was selected, which will be used for breeding Kazakhstan rice varieties with colored pericarp.

摘要

该研究旨在开发用于育种的哈萨克斯坦有色稻壳水稻品种的预育种资源。在研究过程中,对用于该工作的育种材料中“有色稻壳”性状的遗传进行了杂交分析。从哈萨克斯坦共和国植物生物学与生物技术研究所的种质资源库中,挑选出了具有有色稻壳的水稻基因型,以及具有重要育种性状且能在该国条件下成熟的白稻品种。使用PCR方法对所选样本进行了Rc(红色稻壳)和Pb(花青素稻壳)基因的等位基因状态鉴定。在选择杂交亲本时,将具有有色稻壳的国外水稻品种(鲁宾、马夫尔、黑米等)用作受体亲本。作为供体,我们使用了适应水稻种植区土壤和气候条件的哈萨克斯坦本地白稻品种(麦迪娜、马尔占、巴卡纳西、帕克利)以及国外品种。测定了所获得的F1水稻杂种的结实能力和不育率。结果,选出了最有前景的预育种材料,将用于培育具有有色稻壳的哈萨克斯坦水稻品种。

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