Punina E O, Machs E M, Krapivskaya E E, Rodionov A V
Genetika. 2017 Feb;53(2):181-91.
Region ITS1–5.8S rDNA–ITS2 is sequenced in 27 varieties of cultivated ornamental peonies, ten of which presumably originate from Paeonia lactiflora, one from P. officinalis, 13 from hybridization of P. lactiflora and P. peregrina, or P. officinalis, and three are Itoh hybrids. Comparative analysis of distribution patterns of polymorphic sites (PS) for the obtained DNA sequences and data from GenBank is carried out. Hypotheses of origin of the studied varieties, except for two, which, as previously assumed, originate from hybridization of P. lactiflora and P. peregrina, are confirmed. It is shown that the sequence ITS1–5.8S rDNA–ITS2 is a good genetic marker for cultivars of the P. lactiflora group and Itoh hybrids, and that the PS distribution patterns in these sequences can provide valuable information on the kinship and origin of individual varieties. However, insufficient knowledge of wild species from the P. officinalis kinship group limits the use of this marker in the study of varieties obtained through interspecific hybridization within the Paeonia section.
对27个栽培观赏牡丹品种的ITS1–5.8S rDNA–ITS2区域进行了测序，其中10个品种可能源自芍药，1个源自药用芍药，13个源自芍药与野牡丹或药用芍药的杂交品种，3个是伊藤杂种。对所得DNA序列的多态性位点（PS）分布模式与来自GenBank的数据进行了比较分析。除了之前假定的两个品种源自芍药与野牡丹的杂交品种外，其他所研究品种的起源假设均得到证实。结果表明，ITS1–5.8S rDNA–ITS2序列是芍药组品种和伊藤杂种的良好遗传标记，这些序列中的PS分布模式可为各个品种的亲缘关系和起源提供有价值的信息。然而，对药用芍药亲缘关系组中野生种的了解不足，限制了该标记在芍药亚科种间杂交所得品种研究中的应用。
