James-Kracke M R, Sloane B F, Shuman H, Somlyo A P
Proc Natl Acad Sci U S A. 1979 Dec;76(12):6461-5. doi: 10.1073/pnas.76.12.6461.
Spherical electron-dense organelles in the perinuclear region of cultured guinea pig aortic smooth muscle cells were identified as lysosomes by their ability to accumulate acridine orange and by cytochemical demonstration of their acid phosphatase content. The number and size of lysosomes increased in subcultured cells. The elemental composition of the lysosomes was quantitated by electron probe analysis of whole freeze-dried cells and of cryosections. In lysosomes at this stage in their development, the sulfur concentration was higher than that in the cytoplasm and the K/Na concentration ratio was similar to that in the cytoplasm.
通过吖啶橙的累积能力以及酸性磷酸酶含量的细胞化学证明，培养的豚鼠主动脉平滑肌细胞核周区域内的球形电子致密细胞器被鉴定为溶酶体。传代培养细胞中溶酶体的数量和大小增加。通过对全冻干细胞和冷冻切片的电子探针分析对溶酶体的元素组成进行定量。在溶酶体发育的这个阶段，其硫浓度高于细胞质中的硫浓度，钾/钠浓度比与细胞质中的相似。
