Somlyo A P, Garfield R E, Chacko S, Somlyo A V
J Cell Biol. 1975 Aug;66(2):425-43. doi: 10.1083/jcb.66.2.425.
The effects of the ionophoric antibiotic X537A on cell structure were studied with phase-contrast, fluorescence, and electron microscopy. X537A induced selective vacuolation of the Golgi apparatus of vascular and intestinal smooth muscle, epithelium, plasma cells, and cultured chick heart and guinea pig vascular smooth muscle cells. The swelling of the Golgi apparatus induced by X537A was reversible in the systems examined for reversibility: vascular smooth muscle and cultured chick heart. Myelin figures were common in the Golgi apparatus vacuolated by X537A. Fluorescence microscopy of cultured cells incubated with X537A showed the characteristic blue X537A fluorescence associated with lipid globules in the cultured cells. Incubation of cultured chick heart cells with X537A reduced the beating rate and, after 24-72 h, abolished the sarcomere pattern. The swelling of the Golgi membranes produced by X537A in cultured vascular smooth muscle was associated with inhibition of D-[6-3H]glucosamine and [35S]sulfate incorporation into glycosaminoglycans.
利用相差显微镜、荧光显微镜和电子显微镜研究了离子载体抗生素X537A对细胞结构的影响。X537A可诱导血管和肠道平滑肌、上皮细胞、浆细胞以及培养的鸡心脏和豚鼠血管平滑肌细胞的高尔基体出现选择性空泡化。在检测可逆性的系统（血管平滑肌和培养的鸡心脏）中，X537A诱导的高尔基体肿胀是可逆的。在被X537A空泡化的高尔基体中，髓鞘样结构很常见。用X537A孵育培养细胞的荧光显微镜检查显示，培养细胞中与脂质小球相关的特征性蓝色X537A荧光。用X537A孵育培养的鸡心脏细胞会降低跳动速率，并且在24 - 72小时后，肌节模式消失。X537A在培养的血管平滑肌中产生的高尔基体膜肿胀与D - [6 - 3H]葡萄糖胺和[35S]硫酸盐掺入糖胺聚糖的抑制有关。
