饲粮含硫氨基酸对断奶仔猪生长性能、肠道形态、酶活性及养分转运载体的影响。
The effects of dietary sulfur amino acids on growth performance, intestinal morphology, enzyme activity, and nutrient transporters in weaning piglets.
机构信息
Animal Nutrition and Human Health Laboratory, School of Life Sciences, Hunan Normal University, Changsha City, Hunan, China.
Chinese Academy of Science, Institute of Subtropical Agriculture, Research Center for Healthy Breeding of Livestock and Poultry, Hunan Engineering and Research Center of Animal and Poultry Science, Changsha City, Hunan, China.
出版信息
J Anim Sci. 2018 Apr 3;96(3):1130-1139. doi: 10.1093/jas/skx003.
Early weaning results in intestinal dysfunction in piglets, while sulfur amino acids (SAA) are involved in improving intestinal functions. We tested a hypothesis that dietary supplementation with SAA can improve intestinal functions of weaning piglets and analyzed the effects of different dietary SAA levels on intestinal functions. A total of 80 piglets (Duroc × Landrace × Yorkshire) were weaned at 21 d of age and randomly assigned to one of the five diets that contained 0.53%, 0.63%, 0.74%, 0.85%, or 0.96% SAA, which corresponded to 70%, 85%, 100%, 115%, or 130% of the SAA:Lys ratio recommended by the National Research Council (2012). The 14 d feeding experiment involved 16 pens per diet and one piglet per pen. Eight randomly selected piglets from each treatment were euthanized for tissue sampling on day 7 and 14 post weaning. Supplementation with SAA led to a rise over time in G:F (linear, P = 0.001; quadratic, P = 0.001). Between day 0 and 14 of treatment, the jejunal crypt depth decreased (linear, P = 0.018; quadratic, P = 0.015), while that of the duodenal villus (linear, P = 0.049) and ileal villus width (linear, P = 0.029; quadratic, P = 0.034) increased. The activities of jejunal alkaline phosphatase (ALP) were quadratically increased (P = 0.040) from day 0 to 14 due to dietary SAA. Dietary SAA also elevated the activities of jejunal lactase (linear, P = 0.003; quadratic, P = 0.004), jejunal sucrase (linear, P = 0.032; quadratic, P = 0.027), and jejunal contents of glutathione (GSH) from day 0 to 7, as well as the activity of jejunal maltase (linear, P = 0.014; quadratic, P = 0.001) between day 0 and 14. During the first wk, dietary SAA linearly increased the amounts of intestinal-type fatty acid-binding protein (I-FABP) (P = 0.048) and SGLT-1 (P = 0.021) and linearly decreased the amount of GLUT2 (P = 0.029) proteins in the jejunum. The abundance of jejunal I-FABP (P = 0.044) and PEPT1 (P = 0.049) protein linearly increased from day 0 to 14 in response to this supplementation. These findings indicate that there is a dose-dependent response to dietary SAA on feed efficiency and intestinal parameters of weanling pigs.
早期断奶会导致仔猪肠道功能障碍，而含硫氨基酸（SAA）可改善肠道功能。我们提出了一个假设，即膳食补充 SAA 可以改善断奶仔猪的肠道功能，并分析了不同膳食 SAA 水平对肠道功能的影响。总共 80 头（杜洛克×长白×约克夏）仔猪在 21 日龄断奶，并随机分配到五种日粮中的一种，日粮中 SAA 的含量分别为 0.53%、0.63%、0.74%、0.85%或 0.96%，分别相当于推荐的 SAA:Lys 比（2012 年）的 70%、85%、100%、115%或 130%。14 天的喂养试验中，每种日粮有 16 个栏位，每个栏位有 1 头仔猪。在断奶后第 7 天和第 14 天，从每个处理中随机选择 8 头仔猪进行组织取样。随着时间的推移，SAA 的补充导致 G:F 升高（线性，P = 0.001；二次，P = 0.001）。在处理的第 0 天到第 14 天之间，空肠隐窝深度降低（线性，P = 0.018；二次，P = 0.015），而十二指肠绒毛（线性，P = 0.049）和回肠绒毛宽度（线性，P = 0.029；二次，P = 0.034）增加。由于膳食 SAA，空肠碱性磷酸酶（ALP）的活性在第 0 天到第 14 天之间呈二次增加（P = 0.040）。膳食 SAA 还提高了空肠乳糖酶（线性，P = 0.003；二次，P = 0.004）、空肠蔗糖酶（线性，P = 0.032；二次，P = 0.027）和空肠谷胱甘肽（GSH）的活性（线性，P = 0.014；二次，P = 0.001），以及空肠麦芽糖酶（线性，P = 0.014；二次，P = 0.001）的活性从第 0 天到第 14 天。在第 1 周，膳食 SAA 线性增加了肠道型脂肪酸结合蛋白（I-FABP）（P = 0.048）和 SGLT-1（P = 0.021）的数量，并线性降低了 GLUT2（P = 0.029）的数量在空肠中的蛋白质含量。空肠 I-FABP（P = 0.044）和 PEPT1（P = 0.049）蛋白的丰度从第 0 天到第 14 天呈线性增加，这是对这种补充的反应。这些发现表明，膳食 SAA 对断奶仔猪的饲料效率和肠道参数有剂量依赖性反应。
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