将胚胎癌的致瘤性作为一种检测方法，用于研究小鼠囊胚对恶性肿瘤的控制。
Tumorigenicity of embryonal carcinoma as an assay to study control of malignancy by the murine blastocyst.
作者信息
Pierce G B, Lewis S H, Miller G J, Moritz E, Miller P
出版信息
Proc Natl Acad Sci U S A. 1979 Dec;76(12):6649-51. doi: 10.1073/pnas.76.12.6649.
A bioassay, based on tumorigenicity, has been developed to determine the mechanism whereby the blastocyst of the mouse controls malignant expression of embryonal carcinoma. The assay is based upon the incidence of tumors obtained when known numbers of cells of the 402AX strain of embryonal carcinoma are injected into strain 129 mice, compared to the incidence obtained when the same number of embryonal carcinoma cells are incorporated into Swiss-Webster blastocysts that are then injected in strain 129 animals. The results indicate that the blastocyst can regulate one embryonal carcinoma cell consistently; it may have a slight effect on three, but it cannot regulate four or five of them. The position of the embryonal carcinoma cell in the blastocyst is important. Regulation occurs if the embryonal carcinoma cell is placed in the blastocoele cavity, but enhancement of tumorigenicity is obtained if it is placed between the zona pellucida and the trophectoderm. By contrast, the blastocyst is unable to regulate a single B-16 melanoma cell placed in the blastocoele cavity, indicating a degree of specificity for the regulatory process.
一种基于致瘤性的生物测定法已被开发出来，以确定小鼠囊胚控制胚胎癌恶性表达的机制。该测定法基于将已知数量的402AX胚胎癌细胞系的细胞注射到129品系小鼠中所获得的肿瘤发生率，与将相同数量的胚胎癌细胞掺入瑞士-韦伯斯特囊胚中，然后注射到129品系动物中所获得的发生率进行比较。结果表明，囊胚能够一致地调节一个胚胎癌细胞；它可能对三个细胞有轻微影响，但不能调节四个或五个细胞。胚胎癌细胞在囊胚中的位置很重要。如果将胚胎癌细胞置于囊胚腔中，就会发生调节，但如果将其置于透明带和滋养外胚层之间，则会获得致瘤性增强。相比之下，囊胚无法调节置于囊胚腔中的单个B-16黑色素瘤细胞，这表明调节过程具有一定程度的特异性。
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