Chen Zhimin, Zhao Yongfeng, Quan Liang, Zhou Haiting, Cao Dong, Hu Changjiang, Li Wenbing, Yang Zhuo
Chengdu University of TCM, Chengdu 611137, China.
Chengdu Institution of Chinese Herbal Medicine, Chengdu 610016, China.
Evid Based Complement Alternat Med. 2017;2017:2830754. doi: 10.1155/2017/2830754. Epub 2017 Dec 11.
To control the quality of by establishing quality standards, this paper increased the contents of extract and volatile oil determination. Meanwhile, the curcumin was selected as the internal marker, and the relative correlation factors (RCFs) of demethoxycurcumin and bisdemethoxycurcumin were established by high performance liquid chromatography (HPLC). The contents of multicomponents were calculated based on their RCFs. The rationality and feasibility of the methods were evaluated by comparison of the quantitative results between external standard method (ESM) and quantitative analysis of multicomponents by single-marker (QAMS). Ethanol extracts ranged from 9.749 to 15.644% and the mean value was 13.473%. The volatile oil ranged from 0.45 to 0.90 mL/100 g and the mean value was 0.66 mL/100 g. This method was accurate and feasible and could provide a reference for further comprehensive and effective control of the quality standard of and its processed products.
为通过建立质量标准来控制质量,本文增加了提取物含量及挥发油测定。同时,选取姜黄素作为内标物,采用高效液相色谱法(HPLC)建立去甲氧基姜黄素和双去甲氧基姜黄素的相对校正因子(RCFs)。基于其RCFs计算多成分含量。通过外标法(ESM)与单标记多成分定量分析(QAMS)定量结果的比较,评估方法的合理性和可行性。乙醇提取物含量为9.749%至15.644%,平均值为13.473%。挥发油含量为0.45至0.90 mL/100 g,平均值为0.66 mL/100 g。该方法准确可行,可为进一步全面有效控制及其加工产品的质量标准提供参考。