Xing Wei, Xiao-di Yang, Xiao-Li Wang, Jiang-Yan Li, Yuan-Yuan Wang, Hui Xia, Xue-Lian Chang, Qiang Fang
Department of Microbiology and Parasitology, Bengbu Medical College, Anhui Key Laboratory of Infection and Immunity, Bengbu 233030, China.
Department of Public Course, Bengbu Medical College, China.
Zhongguo Xue Xi Chong Bing Fang Zhi Za Zhi. 2016 May 12;28(4):406-410. doi: 10.16250/j.32.1374.2016012.
To understand the status of resistance to multiple insecticides and the frequencies of mutations in from north-central Anhui Province.
From July to September, 2014, the mosquito larvae were collected in Huaibei, Bengbu and Chuzhou cities of the north-central Anhui Province and reared to adults. The female adult mosquitoes at 3-5 days post emergence were tested for susceptibility to the four insecticides, namely 0.05% deltamethrin, 5% malathion, 0.1% bendiocarb and 4% DDT, by using the standard WHO resistance tube bioassay. The detection of the point mutations of the gene at codon 1014 was conducted by PCR and DNA sequencing in the deltamethrin-resistant and -susceptible mosquitoes.
High levels of resistance to all the four insecticides were found in all the three tested populations, although mosquito mortality varied among populations and test insecticides. Among the test insecticides, DDT showed lowest mortality with no significant difference ( = 1.027, > 0.05) in all test populations, whereas significantly different mortalities were observed among populations for the remained three insecticides tested (deltamethrin, malathion, and bendiocarb) ( = 23.823, 33.955, 128.841; all < 0.01). Two types of non-synonymous mutation at codon position 1014 (L1014F and L1014S) were observed. A positive correlation between L1014F mutation frequencies and deltamethrin resistance levels were detected in the three mosquito populations ( = 0.718, < 0.01).
The observed high levels of resistance to multiple-insecticides coupled with the occurrence of medium to high frequencies in populations of could profoundly affect the mosquito vector control programme in China. The local health departments need to strengthen vector dynamic monitoring and implement rational resistance management strategies.
了解皖北中部地区淡色库蚊对多种杀虫剂的抗药性状况及基因突变频率。
2014年7至9月，在皖北中部的淮北、蚌埠和滁州三市采集淡色库蚊幼虫并饲养至成虫。羽化后3至5日龄的雌蚊，采用WHO标准抗性管生物测定法检测其对0.05%溴氰菊酯、5%马拉硫磷、0.1%仲丁威和4%滴滴涕这四种杀虫剂的敏感性。通过PCR和DNA测序对溴氰菊酯抗性和敏感的淡色库蚊进行1014位密码子的基因突变检测。
在所有三个测试种群中均发现对所有四种杀虫剂的高水平抗性，尽管不同种群和测试杀虫剂之间蚊虫死亡率有所差异。在测试的杀虫剂中，滴滴涕在所有测试种群中的死亡率最低，无显著差异（F = 1.027，P > 0.05），而其余三种测试杀虫剂（溴氰菊酯、马拉硫磷和仲丁威）在不同种群间观察到显著不同的死亡率（F = 23.823、33.955、128.841；P均< 0.01）。在1014位密码子处观察到两种非同义突变类型（L1014F和L1014S）。在三个蚊虫种群中检测到L1014F突变频率与溴氰菊酯抗性水平呈正相关（r = 0.718，P < 0.01）。
观察到的对多种杀虫剂的高水平抗性以及淡色库蚊种群中中高频率的基因突变可能会深刻影响中国的蚊媒控制项目。当地卫生部门需要加强媒介动态监测并实施合理的抗性管理策略。
