Chao Li, Ying Zhang, Ting Xiao
Rizhao Municipal Center for Disease Control and Prevention, Shandong Province, Rizhao 276826, China.
Shandong Institute of Parasitic Diseases, China.
Zhongguo Xue Xi Chong Bing Fang Zhi Za Zhi. 2016 Jan 25;28(4):475-477. doi: 10.16250/j.32.1374.2015201.
To diagnose the first imported case of infection by laboratory detection.
The epidemiological data and blood samples of the case were collected, and the samples were detected by the microscopic examination, rapid diagnostic test (RDT) and nested PCR.
The patient was a construction worker backing from Congo, Africa. He experienced the symptoms of irregular fever and weakness one month after returning in Lingyang Town, Junxian County. The results of RDT only suggested no- infection. Under the microscope, it was seen that the infected RBC were obviously disfigured and in irregular shape, the ring forms were thick and big, and also thick granulas in big trophozoite stage and schizont stage were found. The results of PCR showed that the size of amplified product was about 800 bp, which was conformed to that of .
Though microscopic examination is the golden standard for malaria diagnosis, as is difficult to be identified under microscope, the microscopic method combined with PCR test can be used for definite diagnosis.
通过实验室检测诊断首例输入性感染病例。
收集该病例的流行病学资料和血液样本，样本经显微镜检查、快速诊断试验（RDT）和巢式聚合酶链反应（PCR）检测。
患者为一名从非洲刚果回国的建筑工人。他在回到浚县灵阳镇一个月后出现不规则发热和乏力症状。RDT结果仅提示未感染。在显微镜下可见，受感染的红细胞明显变形且形状不规则，环状体粗大，在大滋养体期和裂殖体期还发现粗大颗粒。PCR结果显示扩增产物大小约为800 bp，与……相符。
虽然显微镜检查是疟疾诊断的金标准，但由于……在显微镜下难以鉴别，因此显微镜检查结合PCR检测可用于明确诊断。
