Woda B A, Padden C
Diabetes. 1986 May;35(5):513-6. doi: 10.2337/diab.35.5.513.
The response of BB diabetes-prone (DP) and W-line non-diabetes-prone rats to the T-cell mitogen concanavalin A (ConA) was measured. The W line was a good responder to ConA, whereas the DP was relatively unresponsive. This unresponsiveness could not be reversed with exogenous interleukin 2 (IL-2). The response of DP rats was enhanced by removing adherent cells. To directly test the response of BB T-cells, they were isolated by flow sorting. These experiments demonstrated that BB T-cells could mount a normal ConA response. The normal function of isolated BB T-cells suggested that they were under suppression. Suppressor activity could not be found in the OX8+ population but was found in the DP-adherent cell population. Adherent cells from the W line were not suppressive at the concentrations used. These results showed that the decreased mitogen responsiveness of BB T-cells was not due to an intrinsic T-cell abnormality but was due, in part, to suppression by adherent cells.
对BB糖尿病倾向（DP）大鼠和W系非糖尿病倾向大鼠对T细胞丝裂原刀豆球蛋白A（ConA）的反应进行了测定。W系对ConA反应良好，而DP大鼠相对无反应。这种无反应不能通过外源性白细胞介素2（IL-2）逆转。去除贴壁细胞可增强DP大鼠的反应。为了直接检测BB T细胞的反应，通过流式分选将它们分离出来。这些实验表明，BB T细胞能够产生正常的ConA反应。分离出的BB T细胞的正常功能表明它们受到抑制。在OX8+群体中未发现抑制活性，但在DP贴壁细胞群体中发现了抑制活性。来自W系的贴壁细胞在所使用的浓度下没有抑制作用。这些结果表明，BB T细胞丝裂原反应性降低不是由于内在的T细胞异常，而是部分由于贴壁细胞的抑制作用。
