Bioinformatics Program, Boston University, Boston, United States.
Program in Bioinformatics and Integrative Biology, University of Massachusetts Medical School, Worcester, United States.
Elife. 2018 Jan 29;7:e31628. doi: 10.7554/eLife.31628.
We report a draft assembly of the genome of Hi5 cells from the lepidopteran insect pest, , assigning 90.6% of bases to one of 28 chromosomes and predicting 14,037 protein-coding genes. Chemoreception and detoxification gene families reveal -specific gene expansions that may explain its widespread distribution and rapid adaptation to insecticides. Transcriptome and small RNA data from thorax, ovary, testis, and the germline-derived Hi5 cell line show distinct expression profiles for 295 microRNA- and >393 piRNA-producing loci, as well as 39 genes encoding small RNA pathway proteins. Nearly all of the W chromosome is devoted to piRNA production, and siRNAs are not 2´--methylated. To enable use of Hi5 cells as a model system, we have established genome editing and single-cell cloning protocols. The genome provides insights into pest control and allows Hi5 cells to become a new tool for studying small RNAs ex vivo.
我们报道了鳞翅目害虫 基因组的 Hi5 细胞的草案组装，将 90.6%的碱基分配到 28 条染色体中的一条上，并预测了 14037 个编码蛋白质的基因。化感作用和解毒基因家族揭示了 的特异性基因扩张，这可能解释了它的广泛分布和对杀虫剂的快速适应。来自胸部、卵巢、睾丸和生殖系衍生的 Hi5 细胞系的转录组和小 RNA 数据显示，295 个 microRNA 和 >393 个 piRNA 产生基因座以及 39 个编码小 RNA 途径蛋白的基因具有不同的表达谱。几乎所有的 W 染色体都专门用于 piRNA 的产生，而 siRNAs 不进行 2´--甲基化。为了使 Hi5 细胞能够作为一种模型系统，我们已经建立了基因组编辑和单细胞克隆的方案。该基因组提供了对害虫控制的深入了解，并使 Hi5 细胞成为研究体外小 RNA 的新工具。
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