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基因敲低诱导昆虫细胞系死亡并导致细胞内钙离子水平升高。

gene knockdown induces insect cell lines death and level increases of intracellular calcium ions.

作者信息

Song Jiqiang, Yu Yanping, Yan Zhichao, Xiao Shan, Zhao Xianxin, Wang Fang, Fang Qi, Ye Gongyin

机构信息

State Key Laboratory of Rice Biology and Breeding & Ministry of Agricultural and Rural Affairs Key Laboratory of Molecular Biology of Crop Pathogens and Insects, Institute of Insect Sciences, Zhejiang University, Hangzhou, China.

Department of Entomology, Nanjing Agricultural University, Nanjing, China.

出版信息

Front Physiol. 2023 Jul 6;14:1217954. doi: 10.3389/fphys.2023.1217954. eCollection 2023.

DOI:10.3389/fphys.2023.1217954
PMID:37485065
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10356983/
Abstract

Chloride intracellular channel (CLIC) is a member of the chloride channel protein family for which growing evidence supports a pivotal role in fundamental cellular events. However, the physiological function of CLIC in insects is still rarely uncovered. The ovary-derived High Five (Hi-5) cell line isolated from the cabbage looper () is widely used in laboratories. Here, we studied both characteristics and functions of CLIC in Hi-5 cells (CLIC). We identified the gene in Hi-5 cells and annotated highly conserved CLIC proteins in most insect species. After RNA interference of , the phenomenon of significantly increased cell death suggests that the CLIC protein is essential for the survival of Hi-5 cells. The same lethal effect was also observed in 9 and Schneider 2 cells after knockdown. Furthermore, we found that this kind of cell death was accompanied by increases in intracellular calcium ions after knockdown with the transcriptomic analyses and the detection of calcium levels. Our results provide insights into insect CLIC as a key factor for cell survival and lay the foundation for the cell death mechanism.

摘要

氯离子细胞内通道（CLIC）是氯离子通道蛋白家族的成员，越来越多的证据支持其在基本细胞活动中起关键作用。然而，CLIC在昆虫中的生理功能仍鲜为人知。从甘蓝夜蛾中分离出的卵巢来源的High Five（Hi-5）细胞系在实验室中被广泛使用。在这里，我们研究了Hi-5细胞中CLIC（CLIC）的特性和功能。我们在Hi-5细胞中鉴定了基因，并注释了大多数昆虫物种中高度保守的CLIC蛋白。在对进行RNA干扰后，细胞死亡显著增加的现象表明CLIC蛋白对Hi-5细胞的存活至关重要。在敲低后，在9和Schneider 2细胞中也观察到了同样的致死效应。此外，通过转录组分析和钙水平检测，我们发现这种细胞死亡伴随着敲低后细胞内钙离子的增加。我们的结果为昆虫CLIC作为细胞存活的关键因素提供了见解，并为细胞死亡机制奠定了基础。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c4e3/10356983/e699403ae196/fphys-14-1217954-g007.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c4e3/10356983/e699403ae196/fphys-14-1217954-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c4e3/10356983/62aee28a82d8/fphys-14-1217954-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c4e3/10356983/6d4bd0658e53/fphys-14-1217954-g002.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c4e3/10356983/e699403ae196/fphys-14-1217954-g007.jpg

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本文引用的文献

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Comput Struct Biotechnol J. 2022 Dec 24;21:688-701. doi: 10.1016/j.csbj.2022.12.040. eCollection 2023.
2
A metazoan-specific C-terminal motif in EXC-4 and Gα-Rho/Rac signaling regulate cell outgrowth during tubulogenesis in C. elegans.后生动物特有的 EXC-4 和 Gα-Rho/Rac 信号的 C 端基序调节线虫管状结构形成过程中的细胞外生。
Development. 2022 Dec 15;149(24). doi: 10.1242/dev.200748. Epub 2022 Dec 14.
3
CLIC4 localizes to mitochondrial-associated membranes and mediates cardioprotection.
CLIC4定位于线粒体相关膜并介导心脏保护作用。
Sci Adv. 2022 Oct 21;8(42):eabo1244. doi: 10.1126/sciadv.abo1244.
4
The oxidoreductase CLIC4 is required to maintain mitochondrial function and resistance to exogenous oxidants in breast cancer cells.氯离子通道蛋白 4（CLIC4）是一种氧化还原酶，对于维持乳腺癌细胞中线粒体的功能和抵抗外源性氧化剂至关重要。
J Biol Chem. 2022 Sep;298(9):102275. doi: 10.1016/j.jbc.2022.102275. Epub 2022 Jul 19.
5
ggmsa: a visual exploration tool for multiple sequence alignment and associated data.ggmsa：一个用于多序列比对及相关数据的可视化探索工具。
Brief Bioinform. 2022 Jul 18;23(4). doi: 10.1093/bib/bbac222.
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Four-Octyl itaconate ameliorates periodontal destruction via Nrf2-dependent antioxidant system.辛二酸辛酯通过 Nrf2 依赖的抗氧化系统改善牙周破坏。
Int J Oral Sci. 2022 May 31;14(1):27. doi: 10.1038/s41368-022-00177-1.
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