Department of Hematology, National Cancer Center Hospital, Chuo-ku, Tokyo, Japan.
Department of Pathology and Clinical Laboratory, National Cancer Center Hospital, Chuo-ku, Tokyo, Japan.
Eur J Haematol. 2018 May;100(5):511-517. doi: 10.1111/ejh.13033. Epub 2018 Mar 1.
We aimed at investigating the relationship between classical Hodgkin lymphoma (cHL), primary mediastinal large B-cell lymphoma (PMBL), and gray zone lymphoma (GZL) with intermediate characteristics between cHL and PMBL, from the perspective of the aberration in programed cell death 1 and the programed death ligands (PDLs) network.
We explored the expression levels of PDLs and chromosomal anomalies in 67 cases: 34 cases with cHL, 20 with PMBL, and 13 with GZL, using immunohistochemical analyses and Fluorescence In Situ Hybridization (FISH).
Twenty-one cHL (62%), 3 PMBL (15%), and 6 GZL (46%) cases showed staining to PD-L1 antibodies in more than 70% of tumor cells. Two cHL (6%), 10 PMBL (50%), and 3 GZL (23%) cases were not stained by PD-L1 antibodies. Patients over 40 years old manifest more frequent expression of PD-L1 in cHL. Proportion of tumors stained by PD-L2 antibody was increased in PMBL. FISH analyses with a PD-L1/PD-L2 probe detected 5 amplification, 1 gain, and 7 polysomy cases in cHL, 1 amplification and 1 polysomy case in GZL, and amplification in 1 PMBL case.
We identified increased staining of PD-L1 in cHL and that of PD-L2 in PMBL. GZL had a pattern similar to that of cHL.
本研究旨在从程序性细胞死亡蛋白 1（PD-1）及其配体（PDLs）网络的异常角度，探讨经典霍奇金淋巴瘤（cHL）、原发性纵隔大 B 细胞淋巴瘤（PMBL）和具有 cHL 和 PMBL 中间特征的灰色区淋巴瘤（GZL）之间的关系。
我们使用免疫组织化学分析和荧光原位杂交（FISH）技术，对 67 例病例（34 例 cHL、20 例 PMBL 和 13 例 GZL）的 PDLs 表达水平和染色体异常进行了研究。
21 例 cHL（62%）、3 例 PMBL（15%）和 6 例 GZL（46%）病例中，超过 70%的肿瘤细胞对 PD-L1 抗体染色阳性。2 例 cHL（6%）、10 例 PMBL（50%）和 3 例 GZL（23%）病例对 PD-L1 抗体不染色。40 岁以上的患者中，cHL 更频繁地表达 PD-L1。PD-L2 抗体染色的肿瘤比例在 PMBL 中增加。用 PD-L1/PD-L2 探针进行 FISH 分析，在 cHL 中检测到 5 例扩增、1 例获得和 7 例多倍体病例，在 GZL 中检测到 1 例扩增和 1 例多倍体病例，在 1 例 PMBL 中检测到扩增。
我们发现 cHL 中 PD-L1 染色增加，PMBL 中 PD-L2 染色增加，GZL 具有与 cHL 相似的模式。
