Department of General Pathology, Federal University of Minas Gerais, Institute of Biological Sciences, Belo Horizonte, Minas Gerais, Brazil.
Department of Physiology and Biophysics, Institute of Biological Sciences, Federal University of Minas Gerais, Belo Horizonte, Minas Gerais, Brazil.
Obesity (Silver Spring). 2018 Mar;26(3):531-539. doi: 10.1002/oby.22102. Epub 2018 Jan 27.
Obesity is a highly prevalent multifactorial metabolic condition in which the need for functional bioengineered substitutes (e.g., scaffolds for tissue engineering) is likely to occur. However, the adverse foreign body response (FBR) that invariably takes place adjacent to implant devices impairing their function is poorly characterized in this condition. This study investigated the influence of obesity on the host response to a synthetic matrix implanted subcutaneously in high-fat-fed obese mice.
Histological analysis of 14-day-old implants was performed to identify collagen deposition, capsule thickness, fibroblast-like cells, foreign body giant cells, and mast cells. In addition, transforming growth factor β1 (TGF-β1) levels in the implants and serum were determined.
All fibrogenic markers (and TGF-β1 levels) increased in the implants of obese mice compared with their nonobese counterparts. Particularly relevant was the fibrous capsule thickness in implants of obese mice (234.2 ± 22.1 µm vs. 109.2 ± 13.4 µm in implants of nonobese animals).
The study results showing that obesity upregulates the main features of the FBR induced by subcutaneous implants in mice may be relevant in understanding biomaterial integration and performance in this condition. This is crucial to the development of strategies to maintain the integrity and function of implantable devices.
肥胖是一种高发的多因素代谢性疾病，在此类疾病中，可能需要功能性生物工程替代品（例如组织工程支架）。然而，在这种情况下，植入设备周围不可避免地发生的不良异物反应（FBR）会损害其功能，目前对此反应的特征描述还不够完善。本研究旨在探讨肥胖对高脂肪喂养肥胖小鼠皮下植入合成基质后宿主反应的影响。
对 14 天龄植入物进行组织学分析，以鉴定胶原蛋白沉积、囊厚度、成纤维细胞样细胞、异物巨细胞和肥大细胞。此外，还测定了植入物和血清中的转化生长因子 β1（TGF-β1）水平。
与非肥胖小鼠相比，肥胖小鼠植入物中的所有纤维生成标志物（和 TGF-β1 水平）均增加。肥胖小鼠植入物中纤维囊厚度（234.2±22.1μm）与非肥胖动物植入物中的纤维囊厚度（109.2±13.4μm）相比，增加尤为显著。
研究结果表明，肥胖症上调了肥胖小鼠皮下植入物诱导的异物反应的主要特征，这可能有助于理解此类疾病中生物材料的整合和性能。这对于开发维持植入设备完整性和功能的策略至关重要。
