Infection and Immunity Program, Biomedicine Discovery Institute and Department of Biochemistry and Molecular Biology, Monash University, Clayton, Victoria, Australia.
Proteomics. 2018 Jun;18(12):e1700464. doi: 10.1002/pmic.201700464. Epub 2018 Mar 7.
Immunopeptidomics employs the use of mass spectrometry to identify and quantify peptides presented on the surface of cells by major histocompatibility complex (MHC; human leukocyte antigen [HLA], in humans) molecules, an essential component of adaptive immunity. Currently, immunopeptidomics follows the same or similar workflows as the more established field of shotgun proteomics, yet inherent differences between these two fields create significant drawbacks for the former. In this viewpoint, we would like to highlight such technical issues and provide suggestions for novel workflows that would increase peptide sequencing coverage, depth, and confidence, collectively enhancing the capabilities of the field of immunopeptidomics.
免疫肽组学采用质谱技术来鉴定和定量主要组织相容性复合体（MHC；人类白细胞抗原 [HLA]，在人类中）分子表面呈现的肽，这是适应性免疫的一个重要组成部分。目前，免疫肽组学遵循与更成熟的鸟枪法蛋白质组学相同或相似的工作流程，但这两个领域之间的固有差异给前者带来了重大的缺陷。在这篇观点文章中，我们希望强调这些技术问题，并为增加肽测序覆盖度、深度和置信度提供新的工作流程建议，从而共同提高免疫肽组学领域的能力。
