Pezeshgi Modarres Hassan, Mofrad Mohammad R, Sanati-Nezhad Amir
BioMEMS and Bioinspired Microfluidic Laboratory, Department of Mechanical and Manufacturing Engineering, University of Calgary, Calgary, Alberta, Canada.
Molecular Cell Biomechanics Laboratory, Departments of Bioengineering and Mechanical Engineering, University of California Berkeley, 208A Stanley Hall, Berkeley, CA, United States of America.
PLoS One. 2018 Jan 29;13(1):e0191222. doi: 10.1371/journal.pone.0191222. eCollection 2018.
Protein thermostability engineering is a powerful tool to improve resistance of proteins against high temperatures and thereafter broaden their applications. For efficient protein thermostability engineering, different thermostability-classified data sources including sequences and 3D structures are needed for different protein families. However, no data source is available providing such data easily. It is the first release of ProtDataTherm database for analysis and engineering of protein thermostability which contains more than 14 million protein sequences categorized based on their thermal stability and protein family. This database contains data needed for better understanding protein thermostability and stability engineering. Providing categorized protein sequences and structures as psychrophilic, mesophilic and thermophilic makes this database useful for the development of new tools in protein stability prediction. This database is available at http://profiles.bs.ipm.ir/softwares/protdatatherm. As a proof of concept, the thermostability that improves mutations were suggested for one sample protein belonging to one of protein families with more than 20 mesophilic and thermophilic sequences and with known experimentally measured ΔT of mutations available within ProTherm database.
蛋白质热稳定性工程是一种强大的工具，可提高蛋白质对高温的抗性，从而拓宽其应用范围。对于高效的蛋白质热稳定性工程，不同的蛋白质家族需要不同的按热稳定性分类的数据源，包括序列和三维结构。然而，目前尚无易于获取此类数据的数据源。ProtDataTherm数据库首次发布，用于蛋白质热稳定性分析和工程，其中包含超过1400万个根据热稳定性和蛋白质家族分类的蛋白质序列。该数据库包含更好地理解蛋白质热稳定性和稳定性工程所需的数据。提供按嗜冷、嗜温和嗜热分类的蛋白质序列和结构，使得该数据库对于开发蛋白质稳定性预测的新工具很有用。该数据库可在http://profiles.bs.ipm.ir/softwares/protdatatherm上获取。作为概念验证，对于一个属于具有20多个嗜温和嗜热序列且在ProTherm数据库中有已知实验测量突变ΔT的蛋白质家族的样本蛋白质，提出了改善突变的热稳定性。
