Key Laboratory of Feed Biotechnology of Ministry of Agriculture & National Engineering Research Center of Biological Feed, Feed Research Institute, Chinese Academy of Agricultural Sciences, Beijing, P.R. China.
J Anim Sci. 2018 Feb 15;96(1):225-235. doi: 10.1093/jas/skx007.
The aim of this study was to investigate how dietary supplementation of tea polyphenols (TP) and tea catechins (TC) affect laying performance, albumen quality, ovomucin composition, and magnum morphology of laying hens in the late phase of production. Two hundred seventy Hy-Line Brown laying hens (64 wk old) were assigned to a basal diet (the control), the basal diet supplemented with 200 mg/kg tea polyphenols (TP200) or 200 mg/kg tea catechins (TC200). Each treatment had 6 replicates with 15 hens each. The feeding trial lasted 10 wks. Over the course of the trial, dietary supplementation with TP200 significantly increased the egg production (EP) and improved the feed conversion ratio (FCR) in wk 6 to 10 and wk 1 to 10 (P < 0.05). The albumen height and the Haugh unit (HU) of hens fed TP200 were higher than those of hens fed the control diet at wks 8 and 10 (P < 0.05). However, there were no significant differences in the albumen height and the HU between the TP200 and TC200 groups (P > 0.05). The SDS-PAGE analysis indicated that bands of the ovomucin fractions in the TP200 group had the highest intensity compared with those of the control and TC200 groups. Compared with the control, there was a significant increase in protein sulfhydryl (SH) content of the albumen in the TP200 group at the end of experiment, while a significant decrease in protein carbonyl content and protein surface hydrophobicity (P < 0.05). There were also obvious increase in the height and width of the primary folds, epithelial cell height, and cilia height of the simple columnar epithelium in the TP200 group compared with the control and TC200 groups (P < 0.05). In conclusion, dietary supplementation with 200 mg/kg TP can improve performance, albumen quality, and magnum morphology of aged hens. In addition, TP rather than TC could improve the health status of the magnum for aged layers.
本研究旨在探讨茶多酚（TP）和茶儿茶素（TC）的膳食补充如何影响产蛋后期母鸡的产蛋性能、蛋品质、卵黏蛋白组成和蛋库形态。将 270 只海兰褐产蛋鸡（64 周龄）分为基础日粮（对照组）、基础日粮添加 200mg/kg 茶多酚（TP200）或 200mg/kg 茶儿茶素（TC200）。每个处理有 6 个重复，每个重复 15 只鸡。试验期为 10 周。在试验过程中，TP200 的添加在第 6 周到第 10 周和第 1 周到第 10 周显著提高了产蛋率（EP），改善了饲料转化率（FCR）（P<0.05）。TP200 组母鸡的蛋白高度和哈夫单位（HU）在第 8 周和第 10 周均高于对照组（P<0.05）。然而，TP200 组和 TC200 组之间的蛋白高度和 HU 没有显著差异（P>0.05）。SDS-PAGE 分析表明，与对照组和 TC200 组相比，TP200 组的卵黏蛋白组分的条带强度最高。与对照组相比，TP200 组在试验结束时蛋清中蛋白质巯基（SH）含量显著增加，而蛋白质羰基含量和蛋白质表面疏水性显著降低（P<0.05）。TP200 组的初级褶皱高度、上皮细胞高度和纤毛高度均明显高于对照组和 TC200 组（P<0.05）。综上所述，日粮中添加 200mg/kg TP 可以提高老龄母鸡的生产性能、蛋品质和蛋库形态。此外，TP 而不是 TC 可以改善老龄蛋鸡的蛋库健康状况。
