Yu J, Chen Y, Zhai L, Zhang L, Xu Y, Wang S, Hu S
Department of Veterinary Medicine, College of Animal Sciences, Zhejiang University, Hangzhou, Zhejiang, 310058, P. R. China.
Department of Veterinary Medicine, College of Animal Sciences, Zhejiang University, Hangzhou, Zhejiang, 310058, P. R. China
Poult Sci. 2015 May;94(5):927-33. doi: 10.3382/ps/pev055. Epub 2015 Feb 22.
Previous investigation demonstrated that oral administration of ginseng stem-leaf saponins in chickens could enhance the immune response. The present study was designed to evaluate the effects of ginseng stem-leaf saponins on oxidative stress induced by cyclophosphamide in chickens. One hundred and twenty chickens were randomly divided into 5 groups. Groups 1 to 4 received intramuscular injection of cyclophosphamide to induce oxidative stress while group 5 was injected with saline solution and served as control. Following administration of cyclophosphamide, groups 1 to 3 were orally administered ginseng stem-leaf saponins at 2.5, 5, and 10 mg/kg BW in drinking water for 7 d, respectively. After that, the spleen, thymus, bursa, and serum were collected to measure the indices of the organs and oxidative parameters. The results showed that ginseng stem-leaf saponins significantly inhibited cyclophosphamide-induced oxidative stress by increasing the organ indices, total antioxidant capacity, and the levels of glutathione, ascorbic acid, and α-tocopherol, while elevating the activity of total superoxide dismutase, catalase, and glutathione peroxidase, as well as decreasing the protein carbonyl content and malondialdehyde. Therefore, ginseng stem-leaf saponins could be a promising agent against oxidative stress in the poultry industry.
先前的研究表明，给鸡口服人参茎叶皂苷可增强免疫反应。本研究旨在评估人参茎叶皂苷对环磷酰胺诱导的鸡氧化应激的影响。120只鸡被随机分为5组。第1至4组肌肉注射环磷酰胺以诱导氧化应激，而第5组注射生理盐水作为对照。在给予环磷酰胺后，第1至3组分别在饮水中口服2.5、5和10 mg/kg体重的人参茎叶皂苷，持续7天。之后，收集脾脏、胸腺、法氏囊和血清以测量器官指标和氧化参数。结果表明，人参茎叶皂苷通过增加器官指数、总抗氧化能力以及谷胱甘肽、抗坏血酸和α-生育酚的水平，显著抑制了环磷酰胺诱导的氧化应激，同时提高了总超氧化物歧化酶、过氧化氢酶和谷胱甘肽过氧化物酶的活性，并降低了蛋白质羰基含量和丙二醛含量。因此，人参茎叶皂苷可能是家禽业中对抗氧化应激的一种有前景的药物。
