School of Biological and Food Engineering, Fuyang Teachers College, Fuyang, 236037, China.
Key Laboratory of Embryo Development and Reproductive Regulation in Anhui, Fuyang, 236037, China.
Sci Rep. 2018 Jan 29;8(1):1761. doi: 10.1038/s41598-018-20175-w.
Mammal embryos can be impaired by mother's excessive ethanol uptake, which induces a higher level of reactive oxygen species (ROS) and interferes in one carbon unit metabolism. Here, our analysis by in vitro culture system reveals immediate effect of ethanol in medium on mouse embryo development presents concentration dependent. A preimplantation embryo culture using medium contained 1% ethanol could impact greatly early embryos development, and harmful effect of ethanol on preimplantation embryos would last during the whole development period including of reducing ratio of blastocyst formation and implantation, and deteriorating postimplantation development. Supplement of 50 μg/ml betaine into culture medium can effectively reduce the level of ROS caused by ethanol in embryo cells and rescue embryo development at each stage damaged by ethanol, but supplement of glycine can't rescue embryo development as does betaine. Results of 5-methylcytosine immunodetection indicate that supplement of betaine into medium can reduce the rising global level of genome DNA methylation in blastocyst cells caused by 1% ethanol, but glycine can't play the same impact. The current findings demonstrate that betaine can effectively rescue development of embryos harmed by ethanol, and possibly by restoring global level of genome DNA methylation in blastocysts.
哺乳动物胚胎可能会受到母体过量摄取乙醇的损害，这会导致更高水平的活性氧（ROS）并干扰一碳单位代谢。在这里，我们通过体外培养系统分析发现，乙醇在培养基中的直接作用呈现出浓度依赖性。使用含有 1%乙醇的培养基进行胚胎前体培养会极大地影响早期胚胎的发育，而且乙醇对胚胎前体的有害影响会持续整个发育过程，包括降低囊胚形成和着床的比例，以及恶化着床后的发育。在培养基中补充 50μg/ml 的甜菜碱可以有效降低乙醇在胚胎细胞中引起的 ROS 水平，并拯救乙醇损害的各个阶段的胚胎发育，但甘氨酸的补充并不能像甜菜碱那样拯救胚胎发育。5-甲基胞嘧啶免疫检测的结果表明，甜菜碱的补充可以降低 1%乙醇引起的囊胚细胞中全基因组 DNA 甲基化水平的升高，但甘氨酸不能发挥相同的作用。目前的研究结果表明，甜菜碱可以有效地挽救受乙醇损害的胚胎的发育，可能是通过恢复囊胚中全基因组 DNA 甲基化水平。
