Li Xin, Jiang Song, Wang Xuhai, Hui Wenqiao, Jia Bin
College of Life Sciences, Shihezi University, Road Beisi, Shihezi 832003, Xinjiang, PR China - College of Animal Science and Technology, Shihezi University, Road Beisi, Shihezi 832003, Xinjiang, PR China.
College of Animal Science and Technology, Shihezi University, Road Beisi, Shihezi 832003, Xinjiang, PR China.
Parasite. 2021;28:15. doi: 10.1051/parasite/2021012. Epub 2021 Mar 5.
Cystic echinococcosis, caused by infection with the larval stage of the cestode Echinococcus granulosus, is a chronic zoonosis. The lifecycle of the E. granulosus parasite includes three consecutive stages that require specific gene regulation or protein expression to survive environmental shifts between definitive hosts and intermediate hosts. The aim of the present study is to screen and analyze the stage differential antigens to be considered for vaccine development against E. granulosus. By using the iTRAQ (isobaric tags for relative and absolute quantification) method, the differentially expressed proteins were selected from the three consecutive developmental stages of E. granulosus: oncosphere, adult tapeworms, and protoscolex. Through a bioinformatics analysis including Clusters of Orthologous Groups (COG), Gene Ontology (GO), and pathway metabolic annotation, we identified some proteins of interest from each stage. The results showed that a large number of differentially expressed proteins (375: oncosphere vs. adult, 346: oncosphere vs. protoscolex, and 391: adult vs. protoscolex) were identified from the three main lifecycle stages. Analysis of the differential protein pathways showed that these differential proteins are mainly enriched in metabolic pathways, Huntington's diseases, Alzheimer's diseases, and ribosome metabolic pathways. Interestingly, among these differential proteins, expression levels of paramyosin, HSP60, HSP70, HSP90, cathepsin L1, cathepsin D, casein kinase, and calmodulin were significantly higher in the oncosphere than in the adult or protoscolex (p < 0.05). We hope our findings will help to identify potential targets for diagnosis or for therapeutic and prophylactic intervention.
囊型包虫病是一种慢性人畜共患病，由细粒棘球绦虫幼虫感染所致。细粒棘球绦虫的生命周期包括三个连续阶段，在终末宿主和中间宿主之间转换环境时，需要特定的基因调控或蛋白质表达才能存活。本研究旨在筛选和分析用于开发抗细粒棘球绦虫疫苗的阶段差异抗原。通过使用iTRAQ（相对和绝对定量的等压标签）方法，从细粒棘球绦虫的三个连续发育阶段：六钩蚴、成虫绦虫和原头蚴中选择差异表达蛋白。通过包括直系同源簇（COG）、基因本体论（GO）和通路代谢注释在内的生物信息学分析，我们从每个阶段鉴定出了一些感兴趣的蛋白质。结果表明，从三个主要生命周期阶段鉴定出大量差异表达蛋白（六钩蚴与成虫相比为375个，六钩蚴与原头蚴相比为346个，成虫与原头蚴相比为391个）。差异蛋白通路分析表明，这些差异蛋白主要富集在代谢通路、亨廷顿病、阿尔茨海默病和核糖体代谢通路中。有趣的是，在这些差异蛋白中，六钩蚴中副肌球蛋白、HSP60、HSP70、HSP90、组织蛋白酶L1、组织蛋白酶D、酪蛋白激酶和钙调蛋白的表达水平显著高于成虫或原头蚴（p<0.05）。我们希望我们的发现将有助于确定诊断或治疗及预防干预的潜在靶点。
