Dowran Razieh, Sarvari Jamal, Moattari Afagh, Fattahi Mohammad-Reza, Ramezani Amin, Hosseini Seyed Younes
Department of Bacteriology & Virology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.
Gastroenterohepatology Research center, Shiraz University of Medical Sciences, Shiraz, Iran.
Gastroenterol Hepatol Bed Bench. 2017 Fall;10(4):272-277.
To evaluate the baseline expression of the immune genes in PBMCs of responder and non-responder patients with chronic Hepatitis C.
Although the contribution of peripheral blood mononuclear cell (PBMC) gene expression in treatment outcome of hepatitis C virus (HCV) infection is supposed, it has remained to be distinctly delineated. The baseline expression of the immune genes inside PBMCs may reflect the responsiveness status following IFN treatment.
Totally, 22 chronic HCV encompasses 10 responders and 12 non-responsive cases enrolled randomly regarding medical records. The PBMCs from the peripheral blood samples were isolated and then incubated for 6 hours in the culture media. The baseline expression of TLR7, SOCS1 and ISG15 was measured by Real time PCR.
The gene expression pattern in PBMCs of both groups showed a similar trend. The expression of SOCS1 and TLR7 genes showed higher levels in non-responder group (P>0.05). The result of ISG15 showed a higher but non-significant expression in the responder group (P>0.05).
The similar pattern of TLR7, SOCS1 and ISG15 expression in the responder and non-responder patients indicated their poor discriminating and predictive value in PBMCs sample.
评估慢性丙型肝炎应答者和无应答者外周血单个核细胞（PBMC）中免疫基因的基线表达。
尽管外周血单个核细胞（PBMC）基因表达对丙型肝炎病毒（HCV）感染治疗结果的影响已被推测，但仍有待明确界定。PBMC内免疫基因的基线表达可能反映干扰素治疗后的应答状态。
根据病历随机纳入22例慢性HCV患者，其中10例应答者和12例无应答者。分离外周血样本中的PBMC，然后在培养基中孵育6小时。通过实时PCR检测TLR7、SOCS1和ISG15的基线表达。
两组PBMC中的基因表达模式显示出相似趋势。SOCS1和TLR7基因在无应答组中的表达水平较高（P>0.05）。ISG15在应答组中的表达较高但无统计学意义（P>0.05）。
应答者和无应答者中TLR7、SOCS1和ISG15的相似表达模式表明它们在PBMC样本中的鉴别和预测价值较差。
