Division of Chemistry and Biological Chemistry, School of Physical and Mathematical Sciences, Nanyang Technological University , 21 Nanyang Link, Singapore , 637371.
Bioconjug Chem. 2018 Feb 21;29(2):245-249. doi: 10.1021/acs.bioconjchem.7b00826. Epub 2018 Feb 2.
We developed an enzyme-free, chemical method to selectively label the epigenetic base, 5-hydroxymethylcytosine (hmC) with versatile sulfinate reagents in aqueous solvent under mild reaction conditions. This method allows efficient single step conjugation of biotin to hmC site in DNA for enrichment and pull down assays.
我们开发了一种酶免化学方法，可在温和的反应条件下，以水溶性亚磺酸盐试剂选择性标记表观遗传碱基 5-羟甲基胞嘧啶（hmC）。该方法可在 DNA 的 hmC 位点上高效地进行一步生物素化反应，用于富集和拉下实验。
