Lewis D E, Barron K S, Miller G P, Rich R R
Surv Synth Pathol Res. 1985;4(3):237-47. doi: 10.1159/000156977.
This review has emphasized several recent advances in our understanding of human lymphocyte biology made possible by mAbs that recognize specific functional subpopulations of lymphocytes, coupled with multiparameter flow-cytometric analysis. In the last 3 years, both the T4+ and T8+ subsets of T cells have been further divided according to function and appearance of specific cell surface antigens. In addition, subpopulations of NK cells with different maturation states and B cells with distinct functional abilities have been discovered. Multiparameter flow-cytometric analysis of lymphocyte subpopulations can provide clues to the pathogenetic mechanisms of immune disorders, as has been demonstrated in AIDS patients and children with JRA. The use of multiparameter flow-cytometric analysis to study human immune responses in vitro has enabled a clearer understanding of the complex lymphoid interactions and, as we believe, may play an important role in the analysis of immune-associated disease processes.
本综述着重介绍了我们在理解人类淋巴细胞生物学方面取得的几项最新进展。这些进展得益于识别淋巴细胞特定功能亚群的单克隆抗体，以及多参数流式细胞术分析。在过去3年中，T细胞的T4 +和T8 +亚群根据特定细胞表面抗原的功能和外观进一步细分。此外，还发现了具有不同成熟状态的NK细胞亚群和具有不同功能能力的B细胞亚群。淋巴细胞亚群的多参数流式细胞术分析可以为免疫紊乱的发病机制提供线索，这在艾滋病患者和幼年类风湿性关节炎患儿中已得到证实。利用多参数流式细胞术分析体外人类免疫反应，能够更清楚地了解复杂的淋巴细胞相互作用，并且我们认为，这可能在免疫相关疾病过程的分析中发挥重要作用。
