Li Zhi-Xing, Zhang Hai-Hua, Lan Dan-Chun, Zhang Hong-Tao, Chen Xiao-Zhuan, Sun Jian
Department of Soft Tissue Traumatology, Shenzhen Hospital of Traditional Chinese Medicine, Shenzhen 518033, Guangdong Province, China.
Department of Massage, Shenzhen Hospital of Traditional Chinese Medicine, Shenzhen 518033, Guangdong Pro-vince.
Zhen Ci Yan Jiu. 2018 Jan 25;43(1):8-13. doi: 10.13702/j.1000-0607.170517.
To observe the effect of electroacupuncture(EA) of "Fenglong" (ST 40) and "Sanyinjiao" (SP 6) on lipid metabolic disorder, insulin resistance (IR) and expression of sterol regulatory element blinding protein-1 (SREBP-1) c and fatty acid synthase (FAS) proteins in the liver tissue in hyperlipidemia rats with IR, so as to reveal its mechanisms underlying improvement of IR.
Forty male SD rats were randomly divided into blank control, model, medication and EA groups (＝8 in each group). The IR model was established by feeding the rat with high-fat diet. Rats of the medication group were treated by intragastric administration of pioglitazone (10 mL/kg). For rats of the EA group, EA (2 Hz/100 Hz，1 mA) was applied to bilateral ST 40 and SP 6, once daily for 14 days. The insulin sensitivity index (ISI) was assessed by calculating 60－120 min glucose infusion rate (GIR 60－120) with euglycemic hyperinsulinemic clamp in reference to Kraegen's and colleagues' methods. Fasting blood samples (10 mL) were collected and analyzed for fasting blood glucose (FBG) using enzyme method, serum fasting insulin(FINS) using ELISA, free fatty acid(FFA) using spectrophotometry, and total triglyceride(TG) and total cholesterol(TC) employing glycerine phosphate oxidase peroxidase (GPO-PAP) assay, low density lipoprotein(LDL), high density lipoprotein(HDL) levels using combined filiter paper activity and lipase activity methods, respectively. The IR level was assessed by calculating homeostatic model assessment of insulin resistance (HOMA-IR) using the formula (FBG×FINS)/22.5. The expression levels of SREBP-1 c and FAS proteins in the liver tissue were detected by Western blot.
Following modeling, the GIR 60－120 and serum HDL were significantly decreased(<0.01), and the HOMA-IR, serum FBG, FINS, FFA, TG, TC and LDL, and the expression levels of hepatic SREBP-1 c and FAS proteins were significantly increased in comparison with the blank control group(<0.01). After the intervention, the decreased GIR 60－120 and serum HDL levels were considerably up-regulated (<0.01), and the increased FBG, FINS, FFA, TG, TC and LDL, and hepatic SREBP-1 c and FAS protein levels were notably down-regulated in both EA and medication groups relevant to the model group (<0.05, <0.01). No significant differences were found between the EA and medication groups in all the indexes mentioned above (>0.05).
EA intervention is able to improve the disorder of lipid metabolism of IR rats, which may be associated with its effects in reducing the expression of SREBP-1 c and FAS proteins and in lowering the synthesis of fatty acid.
观察电针“丰隆”（ST 40）、“三阴交”（SP 6）对胰岛素抵抗（IR）高脂血症大鼠脂质代谢紊乱、胰岛素抵抗及肝组织中固醇调节元件结合蛋白-1（SREBP-1）c和脂肪酸合酶（FAS）蛋白表达的影响，以揭示其改善IR的作用机制。
40只雄性SD大鼠随机分为空白对照组、模型组、药物组和电针组（每组8只）。采用高脂饮食喂养建立IR模型。药物组大鼠给予吡格列酮灌胃（10 mL/kg）。电针组大鼠双侧ST 40、SP 6给予电针刺激（2 Hz/100 Hz，1 mA），每日1次，共14天。参照Kraegen等的方法，采用正常血糖高胰岛素钳夹技术计算60～120 min葡萄糖输注速率（GIR 60～120）评估胰岛素敏感性指数（ISI）。采集空腹血样（10 mL），采用酶法检测空腹血糖（FBG），ELISA法检测血清空腹胰岛素（FINS），分光光度法检测游离脂肪酸（FFA），甘油磷酸氧化酶-过氧化物酶（GPO-PAP）法检测总甘油三酯（TG）和总胆固醇（TC），分别采用滤纸结合活性法和脂肪酶活性法检测低密度脂蛋白（LDL）、高密度脂蛋白（HDL）水平。采用公式（FBG×FINS）/22.5计算稳态模型评估胰岛素抵抗（HOMA-IR）评估IR水平。采用Western blot法检测肝组织中SREBP-1 c和FAS蛋白的表达水平。
造模后，与空白对照组比较，模型组大鼠GIR 60～120和血清HDL显著降低（<0.01），HOMA-IR、血清FBG、FINS、FFA、TG、TC和LDL以及肝组织SREBP-1 c和FAS蛋白表达水平显著升高（<0.01）。干预后，与模型组比较，电针组和药物组大鼠降低的GIR 60～120和血清HDL水平明显上调（<0.01），升高的FBG、FINS、FFA、TG、TC和LDL以及肝组织SREBP-1 c和FAS蛋白水平明显下调（<0.05，<0.01）。上述各指标电针组与药物组比较，差异均无统计学意义（>0.05）。
电针干预可改善IR大鼠脂质代谢紊乱，其机制可能与降低SREBP-1 c和FAS蛋白表达、减少脂肪酸合成有关。
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