Jia Ziru, Liu Hongying, Li Wang, Xie Dandan, Cheng Ke, Pi Xitian
Key Laboratory of Biorheology Science and Technology, Ministry of Education, College of Bioengineering, Chongqing University, Chongqing School of Automation & Information Engineering, Sichuan University of Science & Engineering, Zigong, Sichuan Province Chongqing Engineering Research Center of Medical Electronics Key Laboratory for National Defense Science and Technology of innovative micro-nano devices and system technology, Chongqing University, Chongqing, China.
Medicine (Baltimore). 2018 Feb;97(5):e9789. doi: 10.1097/MD.0000000000009789.
In recent years, noninvasive diagnosis based on biomarkers in exhaled breath has been extensively studied. The procedure of biomarker collection is a key step. However, the traditional condenser method has low efficacy in collecting nonvolatile compounds especially the protein biomarkers in breath. To solve this deficiency, here we propose an electret filter method.Exhaled breath of 6 volunteers was collected with a glass condenser and an electret filter. The amount of albumin was analyzed. Furthermore, the difference of exhaled albumin between smokers and nonsmokers was evaluated.The electret filter method collected more albumin than the glass condenser method at the same breath volume level (P < .01). Smokers exhaling more albumin than nonsmokers were also observed (P < .01).The electret filter is capable of collecting proteins more effectively than the condenser method. In addition, smokers tend to exhale more albumin than nonsmokers.
近年来，基于呼出气中生物标志物的非侵入性诊断受到了广泛研究。生物标志物收集过程是关键步骤。然而，传统的冷凝法在收集非挥发性化合物尤其是呼出气中的蛋白质生物标志物方面效率较低。为解决这一不足，我们在此提出一种驻极体过滤法。使用玻璃冷凝器和驻极体过滤器收集了6名志愿者的呼出气。分析了白蛋白含量。此外，评估了吸烟者和非吸烟者呼出气中白蛋白的差异。在相同呼气量水平下，驻极体过滤法比玻璃冷凝器法收集到更多白蛋白（P<0.01）。还观察到吸烟者呼出的白蛋白比非吸烟者多（P<0.01）。驻极体过滤器比冷凝器法更能有效地收集蛋白质。此外，吸烟者呼出的白蛋白往往比非吸烟者多。
