Occupational and Environmental Medicine, Sahlgrenska Academy, University of Gothenburg, Box 414, 405 30 Göteborg, Sweden.
Respir Med. 2012 Feb;106(2):197-204. doi: 10.1016/j.rmed.2011.10.008. Epub 2011 Nov 18.
In this study we test the hypothesis that endogenous particles in exhaled air (PEx), non-invasively sampled from lower airways, are well suited for the analysis of respiratory tract lining fluid (RTLF) proteins, i.e., surfactant protein A (SP-A) and albumin. Ten healthy volunteers were included in the study and participated in two sampling sessions. Blood, exhaled breath condensate (EBC) and PEx were collected at each session. 100 L of breath were collected for each exhaled sample. Serum and exhaled samples were analyzed for SP-A using an in-house ELISA. Albumin was analyzed in exhaled samples using a commercial ELISA kit. SP-A detection rates were 100%, 21%, and 89% for PEx, EBC and serum, respectively. Albumin was detected in PEx, but not in EBC. SP-A measurements in PEx showed good repeatability with an intra-individual coefficient of variation of 13%. Both SP-A and albumin showed significant correlation to mass of PEx (r(s) = 0.93, p < 0.001 and r(s) = 0.86, p = 0.003, respectively). Sampling and analysis of PEx is a valid non-invasive method to monitor RTLF proteins sampled from the lower respiratory tract, as demonstrated here by example of SP-A and albumin analysis.
在这项研究中，我们检验了这样一个假设，即从下呼吸道非侵入性采集的呼出空气中的内源性颗粒（PEx）非常适合分析呼吸道衬里液（RTLF）蛋白，即表面活性蛋白 A（SP-A）和白蛋白。本研究纳入了 10 名健康志愿者，并进行了两次采样。每次采样时均采集血液、呼出气冷凝液（EBC）和 PEx。每次呼气采集 100 L 气体。使用内部 ELISA 法分析血清和呼出气样本中的 SP-A。使用商业 ELISA 试剂盒分析呼出气样本中的白蛋白。PEx、EBC 和血清中 SP-A 的检测率分别为 100%、21%和 89%。PEx 中检测到白蛋白，但 EBC 中未检测到白蛋白。PEx 中 SP-A 的测量具有良好的重复性，个体内变异系数为 13%。SP-A 和白蛋白均与 PEx 质量呈显著相关（r(s) = 0.93，p < 0.001 和 r(s) = 0.86，p = 0.003）。本研究通过对 SP-A 和白蛋白分析的示例，证明了采集和分析 PEx 是一种有效的、非侵入性的方法，可用于监测下呼吸道采集的 RTLF 蛋白。
