Takakuwa K, Kanazawa K, Takeuchi S
Am J Reprod Immunol Microbiol. 1986 Jan;10(1):1-9. doi: 10.1111/j.1600-0897.1986.tb00001.x.
To elucidate an immunological mechanism in terms of the effectiveness of vaccination of the unexplained habitual aborters with their husband's lymphocytes, 20 patients were selected as the experimental group, and 10 fertile couples were selected as the control group. Subjects were studied by mixed lymphocyte reaction-blocking assay so as to determine whether they had blocking antibodies (BAbs) in their sera. In the experimental group, BAb levels were found to be significantly lower as compared to the ten control multiparous wives; 17 cases (85%) of them were proved not to have sufficient BAbs. Significant sharing of HLA-D/DR antigens was observed in experimental couples. Then, 10 out of 17 patients with evidence of no produced BAbs were vaccinated with their husband's lymphocytes, and all of them were observed to produce BAbs within three vaccinations. After vaccination, seven out of ten patients have so far become pregnant, five of these pregnancies have been successful, with evidence of continuing production of BAbs. In conclusion, vaccination of the patients with husband's lymphocytes was found to stimulate a production of BAbs, and it was thus strongly suggested that this may lead to their production in a subsequent pregnancy, which would allow its success.
为了从用丈夫淋巴细胞对不明原因习惯性流产者进行疫苗接种的有效性方面阐明一种免疫机制，选择了20例患者作为实验组，10对有生育能力的夫妇作为对照组。通过混合淋巴细胞反应阻断试验对受试者进行研究，以确定他们血清中是否存在阻断抗体（BAbs）。在实验组中，发现BAb水平与10名对照经产妇相比显著降低；其中17例（85%）被证实没有足够的BAbs。在实验组夫妇中观察到HLA-D/DR抗原的显著共享。然后，对17例无BAbs产生证据的患者中的10例用其丈夫的淋巴细胞进行疫苗接种，观察到所有患者在三次接种内均产生了BAbs。接种疫苗后，到目前为止，10名患者中有7名怀孕，其中5例妊娠成功，有持续产生BAbs的证据。总之，发现用丈夫的淋巴细胞对患者进行疫苗接种可刺激BAbs的产生，因此强烈提示这可能导致其在随后的妊娠中产生，从而使妊娠成功。
