Department of Animal Sciences and Industry, College of Agriculture, Kansas State University, Manhattan, KS.
Department of Diagnostic Medicine/Pathobiology, College of Veterinary Medicine, Kansas State University, Manhattan, KS.
J Anim Sci. 2018 Mar 6;96(2):570-578. doi: 10.1093/jas/sky001.
A total of 360 pigs (DNA 200 × 400, initially 5.9 ± 0.1 kg) were used in a 42 d trial to determine the effect of high doses of a recently available phytase source (Natuphos E 5000 G, BASF Corporation, Florham Park, NJ) on nursery pig growth and bone ash. Pigs were randomly allotted to pens at weaning by BW and pens were allotted to one of eight corn-soybean meal-based dietary treatments in a randomized complete block design. There were five pigs per pen and nine pens per treatment. Diets were fed in three phases (d 0 to 7, 7 to 21, and 21 to 42) with formulated total calcium:phosphorus (Ca:P) of 1.07, 1.05, and 0.93, respectively. Treatments included a negative control (NC) with 0.40, 0.30, or 0.25% aP from monocalcium P for Phases 1, 2, and 3 respectively; and NC with either 500, 1,000, 2,000, 3,000, or 4,000 FTU/kg phytase. The last two treatments were a positive control (PC) with 0.55, 0.45, or 0.40% aP from monocalcium P for Phases 1, 2, and 3, respectively, or PC with 2,000 FTU/kg phytase. The NC diet with 500 FTU/kg and PC without added phytase were formulated to be equivalent in available Ca and P. On d 42, one pig per pen was euthanized and the right fibula was removed for bone ash analysis. From d 0 to 42, pigs fed increasing phytase in the NC tended to have increased (quadratic, P = 0.064) ADG and (linear, P = 0.082) ending BW and had improved (quadratic, P = 0.008) G:F. Adding 2,000 FTU/kg phytase to the PC did not influence ADG or ADFI, but tended to improve (P = 0.060) G:F compared with the PC. In addition, percentage bone ash increased as phytase increased in the NC (linear, P < 0.001) or when 2,000 FTU/kg was added to the PC diets (P < 0.001). Pigs fed the PC had increased (P = 0.007) ADFI and tended to have greater (P = 0.099) percentage bone ash than pigs fed NC+500 FTU/kg phytase, but the pigs fed NC+500 FTU/kg phytase had improved (P = 0.032) G:F compared to pigs fed the PC. In summary, increasing concentrations of dietary phytase in a P-deficient diet improved growth and bone ash measurements, and was optimized at 1,000 FTU/kg. There were varied improvements when 2,000 FTU/kg phytase was added in P adequate diets.
共有 360 头猪（DNA 200×400，初始体重 5.9±0.1kg）参与了为期 42 天的试验，以确定最近可用的植酸酶源（Natuphos E 5000 G，BASF Corporation，Florham Park，NJ）对仔猪生长和骨灰灰分的影响。猪在断奶时按体重随机分配到围栏中，然后按围栏分配到基于玉米-豆粕的八种饮食处理之一的随机完全块设计中。每个围栏有 5 头猪，每个处理有 9 个围栏。日粮分为三个阶段（0 至 7 天、7 至 21 天和 21 至 42 天），配方总钙：磷（Ca：P）分别为 1.07、1.05 和 0.93。处理包括负对照（NC），第 1、2 和 3 阶段的磷分别来自单磷酸钙的 0.40、0.30 和 0.25%；以及 NC 与 500、1000、2000、3000 或 4000 FTU/kg 植酸酶。后两种处理是阳性对照（PC），第 1、2 和 3 阶段的磷分别来自单磷酸钙的 0.55、0.45 或 0.40%，或 PC 与 2000 FTU/kg 植酸酶。添加 500 FTU/kg 植酸酶的 NC 日粮和不添加植酸酶的 PC 日粮在有效钙和磷方面是等效的。在第 42 天，每个围栏的一头猪被安乐死，右侧腓骨被取出用于骨灰灰分分析。从第 0 天到第 42 天，在 NC 中添加植酸酶的猪的 ADG 和终末 BW 呈增加趋势（二次，P=0.064），G:F 得到改善（二次，P=0.008）。在 PC 中添加 2000 FTU/kg 植酸酶不会影响 ADG 或 ADFI，但与 PC 相比，G:F 有改善的趋势（P=0.060）。此外，随着 NC 中植酸酶的增加，骨灰灰分的百分比呈线性增加（P<0.001），或者当在 PC 日粮中添加 2000 FTU/kg 植酸酶时（P<0.001）。与饲喂 NC+500 FTU/kg 植酸酶的猪相比，饲喂 PC 的猪的 ADFI 增加（P=0.007），且骨灰灰分的百分比有增加的趋势（P=0.099），但与饲喂 PC 的猪相比，饲喂 NC+500 FTU/kg 植酸酶的猪的 G:F 改善（P=0.032）。总之，在缺乏磷的日粮中增加植酸酶的浓度可改善生长和骨灰灰分的测量值，最佳浓度为 1000 FTU/kg。在磷充足的日粮中添加 2000 FTU/kg 植酸酶可带来不同程度的改善。
