Department of Animal Sciences and Industry, College of Agriculture, Kansas State University, Manhattan, KS 66506-0201, USA.
Department of Diagnostic Medicine/Pathobiology, College of Veterinary Medicine, Kansas State University, Manhattan, KS 66506-0201, USA.
J Anim Sci. 2023 Jan 3;101. doi: 10.1093/jas/skad306.
A total of 297 pigs (DNA 241 × 600; initially 8.64 ± 0.181 kg) were used in a 21-d trial to determine the efficacy of a novel phytase derived from Citrobacter braakii and expressed in Aspergillis oryzae (HiPhorius; DSM Nutritional Products, Animal Nutrition & Health, Parsippany, NJ) on pig growth and bone mineralization indicators. Pens of pigs were assigned to 1 of 5 dietary treatments in a randomized complete block design with 5 pigs per pen and 12 pens per treatment. The trial was initiated 14-d after weaning. The first three treatments were formulated to contain 0.09% aP; without added phytase (control), or the control diet with 600 or 1,000 FYT/kg of added phytase (considering a release of 0.15% or 0.18% aP, respectively). The remaining two treatments were formulated to contain 0.27% aP, one without added phytase and the other with 1,000 FYT/kg. From days 0 to 21, pigs fed increasing phytase in diets containing 0.09% aP had increased (linear, P ≤ 0.002) ADG, ADFI, and G:F, but added phytase in the 0.27% aP diet did not impact growth performance. Increasing phytase in diets containing 0.09% aP increased percentage bone ash in metacarpals and 10th ribs (linear, P < 0.001; quadratic, P = 0.004, respectively), and increased grams of Ca and P in metacarpals, 10th ribs, and fibulas (linear, P ≤ 0.027). Adding 1,000 FYT/kg phytase in diets with 0.27% aP increased (P ≤ 0.05) percentage bone ash and grams of Ca and P in fibulas and 10th ribs compared with pigs fed 0.27% aP without added phytase. Increasing aP from 0.09% to 0.27% in diets without added phytase increased (P < 0.001) ADG, ADFI, and G:F. Increasing aP from 0.09% to 0.27% in diets without added phytase increased bone density (P ≤ 0.002) in fibulas and metacarpals, percentage bone ash in all bones (P ≤ 0.074), and increased (P < 0.05) grams of Ca and P in fibulas and 10th ribs. Pigs fed diets containing 0.09 or 0.27% aP, both with 1,000 FYT added phytase, had increased (P < 0.05) bone density in fibulas and metacarpals, percentage bone ash in all bones, and increased grams of Ca and P in fibulas and 10th ribs. For growth performance (average of ADG and G:F), aP release was calculated to be 0.170% for 600 FYT/kg and 0.206% for 1,000 FYT/kg. For the average of all bone measurements (average of 3 bones for both bone density and percentage bone ash), aP release was calculated to be 0.120% and 0.125% for 600 and 1,000 FYT/kg, respectively.
共有 297 头猪（DNA 241×600；初始体重 8.64±0.181kg）参与了为期 21 天的试验，以确定来源于柠檬酸杆菌（Citrobacter braakii）并在米曲霉（Aspergillus oryzae）中表达的新型植酸酶（HiPhorius；DSM 营养产品，动物营养与健康，帕西帕尼，新泽西州）对猪生长和骨骼矿化指标的功效。猪舍的猪被分配到 5 种日粮处理中的 1 种，采用随机完全区组设计，每个猪舍 5 头猪，每个处理 12 个猪舍。试验于断奶后 14 天开始。前三种处理配方中含有 0.09%有效磷（AP）；不添加植酸酶（对照），或添加 600 或 1000 FYT/kg 植酸酶的对照日粮（分别考虑释放 0.15%或 0.18%AP）。其余两种处理配方含有 0.27%AP，一种不含添加植酸酶，另一种含有 1000 FYT/kg。从第 0 天到第 21 天，日粮中添加不同水平植酸酶的猪日增重（ADG）、采食量（ADFI）和饲料转化率（G:F）均有所增加（线性，P≤0.002），但 0.27%AP 日粮中添加植酸酶对生长性能没有影响。日粮中添加 0.09%AP 的不同水平植酸酶增加了掌骨和第 10 肋骨的灰分百分比（线性，P<0.001；二次，P=0.004），以及掌骨、第 10 肋骨和腓骨的钙和磷含量（线性，P≤0.027）。日粮中添加 0.27%AP 且添加 1000 FYT/kg 植酸酶增加了第 10 肋骨和腓骨的灰分百分比（P≤0.05）和钙、磷含量。与不添加植酸酶的 0.27%AP 组相比，日粮中不添加植酸酶且添加 0.09%和 0.27%AP 均提高了 ADG、ADFI 和 G:F。日粮中不添加植酸酶且添加 0.09%和 0.27%AP 均增加了腓骨和掌骨的骨密度（P≤0.002）、所有骨骼的灰分百分比（P≤0.074）以及腓骨和第 10 肋骨的钙和磷含量（P<0.05）。饲喂含有 0.09%或 0.27%AP 且添加 1000 FYT/kg 植酸酶日粮的猪，腓骨和掌骨的骨密度（P<0.05）、所有骨骼的灰分百分比以及腓骨和第 10 肋骨的钙和磷含量均有所增加。对于生长性能（ADG 和 G:F 的平均值），600 FYT/kg 植酸酶的磷释放量计算为 0.170%，1000 FYT/kg 植酸酶的磷释放量计算为 0.206%。对于所有骨骼测量的平均值（3 根骨骼的骨密度和灰分百分比的平均值），600 和 1000 FYT/kg 植酸酶的磷释放量分别计算为 0.120%和 0.125%。
