Park Soyoon, Moon Keumok, Park Cheon-Seok, Jung Dong-Hyun, Cha Jaeho
Department of Microbiology, Pusan National University, Busan 46241, Republic of Korea.
Graduate School of Biotechnology and Institute of Life Sciences & Resources, Kyung Hee University, Yongin 17104, Republic of Korea.
J Microbiol Biotechnol. 2018 Feb 1. doi: 10.4014/jmb.1711.11055.
Because glycosylation of aesculetin and its 6-glucoside, aesculin, enhances their biological activities and physicochemical properties, whole-cell biotransformation and enzymatic synthesis methodologies using amylosucrase were compared to determine the optimal production method for glycoside derivatives. High performance liquid chromatography analysis of reaction products revealed two glycosylated products (AGG1 and AGG2) when aesculin was used as an acceptor and three products (AG1, AG2, and AG3) when using aesculetin. The whole-cell biotransformation production yields of the major transfer products for each acceptor (AGG1 and AG1) were 85% and 25%, respectively, compared to 68% and 14% for enzymatic synthesis. These results indicate that whole-cell biotransformation is more efficient than enzymatic synthesis for the production of glycoside derivatives.
