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泰山松花粉多糖的初步表征、抗氧化和保肝活性。

Preliminary Characterization, Antioxidant and Hepatoprotective Activities of Polysaccharides from Taishan Pinus massoniana Pollen.

机构信息

College of Animal Science and Veterinary Medicine, Shandong Agricultural University, Tai'an 271018, China.

College of Veterinary Medicine, Yangzhou University, 12 East Wenhui Road, Yangzhou 225009, China.

出版信息

Molecules. 2018 Jan 30;23(2):281. doi: 10.3390/molecules23020281.

DOI:10.3390/molecules23020281
PMID:29385683
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6017409/
Abstract

The objectives of the present study were to characterize the chemical composition, antioxidant activity and hepatoprotective effect of the polysaccharides from Taishan pollen (TPPPS). HPLC analysis showed that TPPPS was an acidic heteropolysaccharide with glucose and arabinose as the main component monosaccharides (79.6%, molar percentage). Fourier transform-infrared spectroscopy (FT-IR) analysis indicated that the spectra of TPPPS displayed infrared absorption peaks characteristic of polysaccharides. In in vitro assays TPPPS exhibited different degrees of dose-dependent antioxidant activities , and this was further verified by suppression of CCl₄-induced oxidative stress in the liver with three tested doses of TPPPS (100, 200, and 400 mg/kg bw) in rats. Pretreatment with TPPPS significantly decreased the levels of alanine aminotransferase (AST), aspartate aminotransferase (ALT), alkaline phosphatase (ALP), lactic dehydrogenase (LDH) and malondialdehyde (MDA) against CCl₄ injuries, and elevated the activities of superoxide dismutase (SOD) as well as glutathione peroxidase (GSH-Px). Histopathological observation further confirmed that TPPPS could protect the liver tissues from CCl₄-induced histological alternation. These results suggest that TPPPS has strong antioxidant activities and significant protective effect against acute hepatotoxicity induced by CCl₄. The hepatoprotective effect may partly be related to its free radical scavenging effect, increasing antioxidant activity and inhibiting lipid peroxidation.

摘要

本研究的目的是研究泰山花粉多糖（TPPPS）的化学成分、抗氧化活性和保肝作用。HPLC 分析表明，TPPPS 是一种酸性杂多糖，主要由葡萄糖和阿拉伯糖组成单糖（79.6%，摩尔百分比）。傅里叶变换红外光谱（FT-IR）分析表明，TPPPS 的光谱显示出多糖的特征红外吸收峰。在体外试验中，TPPPS 表现出不同程度的剂量依赖性抗氧化活性，这进一步通过用三种不同剂量的 TPPPS（100、200 和 400 mg/kg bw）在大鼠中抑制 CCl₄诱导的肝氧化应激得到证实。TPPPS 预处理显著降低了丙氨酸氨基转移酶（AST）、天冬氨酸氨基转移酶（ALT）、碱性磷酸酶（ALP）、乳酸脱氢酶（LDH）和丙二醛（MDA）在 CCl₄损伤下的水平，并提高了超氧化物歧化酶（SOD）和谷胱甘肽过氧化物酶（GSH-Px）的活性。组织病理学观察进一步证实，TPPPS 可以保护肝组织免受 CCl₄诱导的组织学改变。这些结果表明，TPPPS 具有很强的抗氧化活性和显著的保护作用，可对抗 CCl₄ 诱导的急性肝毒性。其保肝作用可能部分与其清除自由基、提高抗氧化活性和抑制脂质过氧化有关。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7959/6017409/19a265060cf6/molecules-23-00281-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7959/6017409/122dc7d278f2/molecules-23-00281-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7959/6017409/a086cc020300/molecules-23-00281-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7959/6017409/d887cef8bfde/molecules-23-00281-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7959/6017409/e0bb151710b8/molecules-23-00281-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7959/6017409/d2215134fa78/molecules-23-00281-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7959/6017409/19a265060cf6/molecules-23-00281-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7959/6017409/122dc7d278f2/molecules-23-00281-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7959/6017409/a086cc020300/molecules-23-00281-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7959/6017409/d887cef8bfde/molecules-23-00281-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7959/6017409/e0bb151710b8/molecules-23-00281-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7959/6017409/d2215134fa78/molecules-23-00281-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7959/6017409/19a265060cf6/molecules-23-00281-g006.jpg

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