Key Laboratory of Forestry Genetics & Biotechnology of Ministry of Education, Co-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing 210037, China.
Wenzhou Key laboratory of Resource Plant Innovation and Utilization, Zhejiang Institute of Subtropical Crops, Wenzhou 325005, China.
Genes (Basel). 2022 Mar 7;13(3):469. doi: 10.3390/genes13030469.
Masson pine () is a core industrial tree species that is used for afforestation in southern China. Previous studies have shown that (s) are involved in the growth and development of various species, but the function of s in Masson pine is unclear. In this research, we cloned and identified Masson pine cDNA (PmARF6). The results showed that encodes a protein of 681 amino acids that is highly expressed in female flowers. Subcellular analysis showed that the PmARF6 protein occurred predominantly in the nucleus and cytomembrane of Masson pine cells. Compared with wild-type (WT) , transgenic plants overexpressing had fewer rosette leaves, and their flower development was slower. These results suggest that overexpression of may inhibit the flower and leaf development of Masson pine and provide new insights into the underlying developmental mechanism.
马尾松（）是中国南方造林的核心工业树种。先前的研究表明， ARF 参与了多种物种的生长和发育，但马尾松 s 的功能尚不清楚。在这项研究中，我们克隆并鉴定了马尾松 cDNA（PmARF6）。结果表明，编码一个由 681 个氨基酸组成的蛋白质，在雌花中高度表达。亚细胞分析表明，PmARF6 蛋白主要存在于马尾松细胞的核和细胞质膜中。与野生型（WT）相比，过表达的转基因植株的莲座叶较少，花的发育也较慢。这些结果表明，过表达可能会抑制马尾松的花和叶发育，并为其潜在的发育机制提供新的见解。
