Javaid J I, Liskevych U
Biomed Environ Mass Spectrom. 1986 Mar;13(3):129-32. doi: 10.1002/bms.1200130306.
This report describes a highly sensitive capillary gas chromatographic/mass spectrometric procedure for the quantification of alprazolam in human plasma. Alprazolam was extracted from plasma at basic pH with benzeneisoamyl alcohol (98:2, v/v) and analysed directly without any derivatization. Triazolam, also a triazolobenzodiazepine derivative, was used as an internal standard because its extraction characteristics were similar to alprazolam. High sensitivity was achieved by negative ion chemical ionization and selected ion monitoring for alprazolam (m/z 308) and triazolam (m/z 306). The within-day coefficient of variation of the method over the concentration range 1.0-20 ng alprazolam per ml of plasma was 5.5%.
本报告描述了一种用于定量测定人血浆中阿普唑仑的高灵敏度毛细管气相色谱/质谱分析方法。阿普唑仑在碱性pH条件下用苯-异戊醇（98:2，v/v）从血浆中萃取，无需任何衍生化即可直接分析。三唑仑也是一种三唑并苯二氮䓬衍生物，因其萃取特性与阿普唑仑相似，用作内标。通过负离子化学电离以及对阿普唑仑（m/z 308）和三唑仑（m/z 306）的选择离子监测实现了高灵敏度。该方法在每毫升血浆中阿普唑仑浓度范围为1.0 - 20 ng时日内变异系数为5.5%。
