使用气相色谱-质谱联用仪（GC-MS）对血液和尿液中的苯二氮䓬类药物进行定量分析。

Quantitation of benzodiazepines in blood and urine using gas chromatography-mass spectrometry (GC-MS).

作者信息

Goldberger Bruce A, Chronister Chris W, Merves Michele L

机构信息

Department of Pathology, Immunology, and Laboratory Medicine, University of Florida College of Medicine, Gainesville, FL, USA.

出版信息

Methods Mol Biol. 2010;603:75-87. doi: 10.1007/978-1-60761-459-3_8.

DOI:10.1007/978-1-60761-459-3_8

PMID:20077061

Abstract

The benzodiazepine assay utilizes gas chromatography-mass spectrometry (GC-MS) for the analysis of diazepam, nordiazepam, oxazepam, temazepam, lorazepam, alpha-hydroxyalprazolam, and alpha-hydroxytriazolam in blood and urine. A separate assay is employed for the analysis of alprazolam. Prior to solid phase extraction, urine specimens are subjected to enzyme hydrolysis. The specimens are fortified with deuterated internal standard and a five-point calibration curve is constructed. Specimens are extracted by mixed-mode solid phase extraction. The benzodiazepine extracts are derivatized with N-methyl-N-(tert-butyldimethylsilyl)trifluoroacetamide (MTBSFTA) producing tert-butyldimethyl silyl derivatives; the alprazolam extracts are reconstituted in methanol without derivatization. The final extracts are then analyzed using selected ion monitoring GC-MS.

摘要

苯二氮䓬检测采用气相色谱-质谱联用（GC-MS）技术分析血液和尿液中的地西泮、去甲地西泮、奥沙西泮、替马西泮、劳拉西泮、α-羟基阿普唑仑和α-羟基三唑仑。阿普唑仑的分析采用单独的检测方法。在固相萃取之前，尿液标本要进行酶水解。标本用氘代内标进行强化，并构建五点校准曲线。标本通过混合模式固相萃取进行提取。苯二氮䓬提取物用N-甲基-N-（叔丁基二甲基硅基）三氟乙酰胺（MTBSFTA）衍生化，生成叔丁基二甲基硅基衍生物；阿普唑仑提取物在甲醇中复溶，无需衍生化。然后使用选择离子监测GC-MS对最终提取物进行分析。