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犬及猕猴(恒河猴和食蟹猴)牙齿暴露于二氧化碳激光束下牙髓牙本质组织制备的实验研究

[Experimental research on the preparation of dentin-pulp tissue of teeth exposed to CO2 laser beams in dogs and macaques (Macaca mulatta and Macaca fascicularis].

作者信息

Melcer J, Chaumette M T, Melcer F, Zeboulon S

出版信息

C R Seances Soc Biol Fil. 1985;179(5):577-85.

PMID:2938685
Abstract

Traumatic inflammation due to application of CO2 laser radiation (lambda = 10.6 microns) on the dentin and on pulp of 342 teeth of 8 dogs (Beagle) and of 6 monkeys (Macaca sp. mulatta et fascicularis) stimulate in dentino-pulpal tissue morphological phenomenon, studied after 5 days, 1 and 3 months by microscopy and microradiography. The dentin exposed to a density of energy of: 2 X 10(3) J/cm2 shows a burn, a rarefaction and a cellular degeneration, followed by a neoformation of calcified dentin, of about 300 microns thick in 3 months, due to the excitation of odontoblasts or produced by pulpal cells functioning before. A density of energy of 10(3) J/cm2 order applied to the pulp beads to its partial necrosis, to various inflammatory aspects (vascular, cellular, tissular) and to a quasi-constant regeneration of by a neo-dentin bridge of 200 micron at 1 month. This method seems able to allow the analysis of cell activity (start, activation, blocking).

摘要

对8只比格犬和6只猕猴(恒河猴和食蟹猴)的342颗牙齿的牙本质和牙髓施加二氧化碳激光辐射(波长 = 10.6微米)所引起的创伤性炎症,会刺激牙髓 - 牙本质组织出现形态学现象,在5天、1个月和3个月后通过显微镜检查和显微放射照相术进行研究。暴露于能量密度为2×10³ J/cm²的牙本质会出现烧伤、稀疏和细胞变性,随后在3个月内会形成约300微米厚的钙化牙本质新形成,这是由于成牙本质细胞的激发或之前牙髓细胞的功能作用所产生的。施加于牙髓珠的能量密度为10³ J/cm²量级会导致其部分坏死、出现各种炎症表现(血管、细胞、组织方面),并在1个月时由200微米的新牙本质桥实现近乎持续的再生。这种方法似乎能够对细胞活性(启动、激活、阻断)进行分析。

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