Melcer J, Chaumette M T, Melcer F
Faculté de Chirurgie-Dentaire, Université Paris-V, Montrouge, France.
Lasers Surg Med. 1987;7(4):347-52. doi: 10.1002/lsm.1900070409.
Traumatic inflammation due to application of CO2 laser beam on teeth (dentin and pulp) of Beagle dogs and Macaca Monkeys, stimulate in dentino-pulpal tissue morphological phenomena studied after 5 days, 1, and 3 months by microscopy and microradiography. After a dentin exposure to a density of energy from 2 X 10(3) J/cm2, the first cell layers of the pulp tissue show a rarefaction and a cellular degeneration, followed by a neoformation of calcified dentin, of about 300 microns thick in 3 months, due to the excitation of odontoblasts or produced by pulpal cells functioning before. A density of energy of 10(3) J/cm2 order applied to the pulp beads to its partial necrosis, to various inflammatory aspects and to a quasi-constant regeneration by a neo-dentin bridge of 200 microns at 1 month. The analysis of cell activity seems able to be investigated from this method.
在比格犬和猕猴的牙齿（牙本质和牙髓）上应用二氧化碳激光束引起的创伤性炎症，通过显微镜和微放射照相术研究了5天、1个月和3个月后牙本质-牙髓组织中的形态学现象。牙本质暴露于2×10³J/cm²的能量密度后，牙髓组织的第一层细胞出现稀疏和细胞变性，随后由于成牙本质细胞的激活或先前发挥功能的牙髓细胞产生的作用，在3个月内形成约300微米厚的钙化牙本质新形成物。将10³J/cm²量级的能量密度施加于牙髓珠，会导致其部分坏死、出现各种炎症表现，并在1个月时通过200微米的新牙本质桥实现近乎恒定的再生。似乎可以从这种方法研究细胞活性分析。
