Mutalib Maisarah Abdul, Rahmat Asmah, Ali Faisal, Othman Fauziah, Ramasamy Rajesh
Department of Nutrition and Dietetics, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, 43400 UPM, Serdang Selangor, Malaysia.
Faculty of Science Technology and Human Development, Universiti Tun Hussein Onn, 86400 Parit Raja, Batu Pahat Johor, Malaysia.
Malays J Med Sci. 2017 Oct;24(5):19-32. doi: 10.21315/mjms2017.24.5.3. Epub 2017 Oct 26.
This study aims to examine various solvent extracts of (tamarillo) also known as the tree tomato, for their bioactive constituents and antioxidant activity. The study also aims to examine its effect on cancer cell death using two types of cancer cell lines (liver and breast cancer cell).
The first part of the study evaluates the nutritional composition of tamarillo. Then, phytochemical profiling using GC-MS analysis in ethanolic tamarillo extract was conducted. Different fractions of n-butanol, ethyl acetate and aqueous fractions were obtained from the ethanolic extract of tamarillo. Then, the fractions were subjected to the quantification of total phenol (TPC) and flavonoid contents (TFC), free radical scavenging activity (SA) and also antioxidant activity (AOX) assayed by beta-carotene bleaching (BCB) assay. Finally, the capability of the ethanolic extract of tamarillo and different fractions were evaluated for their anticancer properties.
Findings from this study revealed that the nutritional composition (ash, protein, carbohydrate and total dietary fiber), and mineral levels (calcium, magnesium, potassium and iron) of tamarillo were moderate. The crude ethanol extract of tamarillo contained the highest phenolic and total flavonoid content. FT-IR analysis revealed the presence of alkanes, carboxylic acid, phenol, alkanes, carboxylic acids, aromatics and nitro compounds. Twelve bioactive constituents in tamarillo have been identified through GC-MS analysis. Cytotoxic activity suggests the potential of ethanolic extracts of tamarillo having a chemopreventive effect on breast and liver cancer cells.
This study reveals that tamarillo has substantial antioxidant activity as well as anticancer properties.
本研究旨在检测树番茄（又称鸡蛋果）的各种溶剂提取物的生物活性成分和抗氧化活性。该研究还旨在检测其对两种癌细胞系（肝癌细胞和乳腺癌细胞）的癌细胞死亡的影响。
研究的第一部分评估了树番茄的营养成分。然后，对树番茄乙醇提取物进行了气相色谱 - 质谱联用（GC - MS）分析的植物化学图谱分析。从树番茄乙醇提取物中获得了正丁醇、乙酸乙酯和水相的不同馏分。接着，对这些馏分进行总酚（TPC）和黄酮含量（TFC）的定量、自由基清除活性（SA）以及通过β - 胡萝卜素漂白（BCB）测定法测定的抗氧化活性（AOX）。最后，评估了树番茄乙醇提取物和不同馏分的抗癌特性。
本研究结果表明，树番茄的营养成分（灰分、蛋白质、碳水化合物和总膳食纤维）以及矿物质含量（钙、镁、钾和铁）适中。树番茄的粗乙醇提取物含有最高的酚类和总黄酮含量。傅里叶变换红外光谱（FT - IR）分析表明存在烷烃、羧酸、酚、烷烃、羧酸、芳烃和硝基化合物。通过GC - MS分析已鉴定出树番茄中的12种生物活性成分。细胞毒性活性表明树番茄乙醇提取物对乳腺癌和肝癌细胞具有化学预防作用的潜力。
本研究表明，树番茄具有显著的抗氧化活性以及抗癌特性。
