School of Graduate Studies (SGS), Management and Science University, Shah Alam 40100, Malaysia.
Pre-Clinical Department, Faculty of Medicine and Defence Health, Universiti Pertahanan Nasional Malaysia, Kuala Lumpur 57000, Malaysia.
Molecules. 2023 Jul 10;28(14):5322. doi: 10.3390/molecules28145322.
The advancement in nanotechnology is the trigger for exploring the synthesis of selenium nanoparticles and their use in biomedicine. Therefore, this study aims to synthesize selenium nanoparticles using as a reducing agent and evaluate their antioxidant and antidiabetic potential. Our result demonstrated a change in the color of the mixture from yellow to red, and UV-Vis spectrometry of the suspension solution confirmed the formation of MO-SeNPs with a single absorbance peak in the range of 240-560 nm wavelength. FTIR analysis revealed several bioactive compounds, such as phenols and amines, that could possibly be responsible for the reduction and stabilization of the MO-SeNPs. FESEM + EDX analysis revealed that the amorphous MO-SeNPs are of high purity, have a spherical shape, and have a size of 20-250 nm in diameter, as determined by HRTEM. MO-SeNPs also exhibit the highest DPPH scavenging activity of 84% at 1000 μg/mL with an IC50 of 454.1 μg/mL and noteworthy reducing ability by reducing power assay. Furthermore, MO-SeNPs showed promising antidiabetic properties with dose-dependent inhibition of α-amylase (26.7% to 44.53%) and α-glucosidase enzyme (4.73% to 19.26%). Hence, these results demonstrated that plant extract possesses the potential to reduce selenium ions to SeNPs under optimized conditions with notable antioxidant and antidiabetic activities.
纳米技术的进步引发了人们对硒纳米粒子的合成及其在生物医学中的应用的探索。因此，本研究旨在使用 作为还原剂合成硒纳米粒子，并评估其抗氧化和抗糖尿病潜力。我们的结果表明，混合物的颜色从黄色变为红色，悬浮液的紫外-可见光谱证实了 MO-SeNPs 的形成，其在 240-560nm 波长范围内具有单一的吸收峰。傅里叶变换红外光谱（FTIR）分析显示，几种生物活性化合物，如酚类和胺类，可能是导致 MO-SeNPs 还原和稳定的原因。FESEM+EDX 分析表明，非晶态 MO-SeNPs 具有高纯度、球形形状，且通过高分辨率透射电子显微镜（HRTEM）确定其直径为 20-250nm。MO-SeNPs 在 1000μg/mL 时对 DPPH 的清除活性最高，达到 84%，IC50 为 454.1μg/mL，且具有显著的还原能力。此外，MO-SeNPs 对α-淀粉酶（26.7%至 44.53%）和α-葡萄糖苷酶（4.73%至 19.26%）的抑制作用呈剂量依赖性，表现出有前景的抗糖尿病特性。因此，这些结果表明， 植物提取物在优化条件下具有将硒离子还原为 SeNPs 的潜力，同时具有显著的抗氧化和抗糖尿病活性。
