Janoska Agnes, Vázquez María, Janssen Marcel, Wijffels René H, Cuaresma María, Vílchez Carlos
AlgaePARC, Bioprocess Engineering, Wageningen University and Research, Wageningen, 6700AA, The Netherlands.
Algal Biotechnology Group, University of Huelva, Edificio CIDERTA, Parque Huelva Empresarial S/N, Huelva, 21007, Spain.
Biotechnol Prog. 2018 May;34(3):711-720. doi: 10.1002/btpr.2614. Epub 2018 Feb 16.
A novel liquid foam-bed photobioreactor has been shown to hold potential as an innovative technology for microalgae production. In this study, a foam stabilizing agent has been selected which fits the requirements of use in a liquid foam-bed photobioreactor. Four criteria were used for an optimal surfactant: the surfactant should have good foaming properties, should not be rapidly biodegradable, should drag up microalgae in the foam formed, and it should not be toxic for microalgae. Ten different surfactants (nonionic, cationic, and anionic) and two microalgae genera (Chlorella and Scenedesmus) were compared on the above-mentioned criteria. The comparison showed the following facts. Firstly, poloxameric surfactants (Pluronic F68 and Pluronic P84) have acceptable foaming properties described by intermediate foam stability and liquid holdup and small bubble size. Secondly, the natural surfactants (BSA and Saponin) and Tween 20 were easily biodegraded by bacteria within 3 days. Thirdly, for all surfactants tested the microalgae concentration is reduced in the foam phase compared to the liquid phase with exception of the cationic surfactant CTAB. Lastly, only BSA, Saponin, Tween 20, and the two Pluronics were not toxic at concentrations of 10 CMC or higher. The findings of this study indicate that the Pluronics (F68 and P84) are the best surfactants regarding the above-mentioned criteria. Since Pluronic F68 performed slightly better, this surfactant is recommended for application in a liquid foam-bed photobioreactor. © 2018 American Institute of Chemical Engineers Biotechnol. Prog., 34:711-720, 2018.
一种新型的液体泡沫床光生物反应器已被证明具有作为微藻生产创新技术的潜力。在本研究中，选择了一种符合液体泡沫床光生物反应器使用要求的泡沫稳定剂。选择最佳表面活性剂使用了四个标准：表面活性剂应具有良好的发泡性能，不应快速生物降解，应将微藻带入形成的泡沫中，并且对微藻无毒。根据上述标准，比较了十种不同的表面活性剂（非离子型、阳离子型和阴离子型）和两种微藻属（小球藻属和栅藻属）。比较结果如下。首先，泊洛沙姆表面活性剂（普朗尼克F68和普朗尼克P84）具有可接受的发泡性能，表现为中等泡沫稳定性、持液量和小气泡尺寸。其次，天然表面活性剂（牛血清白蛋白和皂角苷）和吐温20在3天内很容易被细菌生物降解。第三，除阳离子表面活性剂十六烷基三甲基溴化铵外，对于所有测试的表面活性剂，与液相相比，泡沫相中微藻浓度降低。最后，只有牛血清白蛋白、皂角苷、吐温20和两种普朗尼克在浓度为10临界胶束浓度或更高时无毒。本研究结果表明，就上述标准而言，普朗尼克（F68和P84）是最佳表面活性剂。由于普朗尼克F68表现稍好，推荐该表面活性剂用于液体泡沫床光生物反应器。©2018美国化学工程师学会生物技术进展，34:711 - 720，2018。
