Scott J E, Hughes E W
Connect Tissue Res. 1986;14(4):267-78. doi: 10.3109/03008208609017470.
Developing and mature flexor digitorum tendons of chick and bovine origin were analyzed for nucleic acid, hydroxyproline, hyaluronate, chondroitin sulfate and sulfated glycosaminoglycan hexuronate. Collagen fibril diameters were determined by electron microscopy. Results are compared with similar data from rat tail tendon. Tissue hydroxyproline (mg/g) increased rapidly in early embryogenesis, while collagen fibril diameters remained relatively constant and small. Hyaluronate and chondroitin sulfate were present in considerable quantities, (phase I). A rapid increase in fibril diameters in midterm 'pregnancy' coincided with a decrease in hyaluronate and chondroitin sulfate contents (phase II). The transition from phase I to phase II was associated with the first loading by active muscles of the tendon, independently of whether the animal was pre- or post partum. The probable existence of a developmental program intrinsic to tendon itself, is discussed. The data are compared with predictions from three different simple models. Hyaluronate is concluded to be entirely interfibrillar, chondroitin sulfate is probably similarly distributed, whereas dermatan sulfate is entirely fibril-surface associated. The 'small' dermatan sulfate proteoglycan is assigned to the 'd' band in the gap zone.
对鸡和牛来源的发育中和成熟的指屈肌腱进行了核酸、羟脯氨酸、透明质酸盐、硫酸软骨素和硫酸化糖胺聚糖己糖醛酸分析。通过电子显微镜测定胶原纤维直径。将结果与来自大鼠尾腱的类似数据进行比较。组织羟脯氨酸（mg/g）在胚胎发育早期迅速增加，而胶原纤维直径保持相对恒定且较小。透明质酸盐和硫酸软骨素大量存在（阶段I）。中期“妊娠”时纤维直径迅速增加，同时透明质酸盐和硫酸软骨素含量减少（阶段II）。从阶段I到阶段II的转变与肌腱首次受到活跃肌肉的负荷有关，与动物是产前还是产后无关。讨论了肌腱本身可能存在的内在发育程序。将数据与三种不同简单模型的预测进行比较。得出结论，透明质酸盐完全存在于纤维间，硫酸软骨素可能分布类似，而硫酸皮肤素完全与纤维表面相关。“小”硫酸皮肤素蛋白聚糖被归为间隙区的“d”带。
