Department of Food Science and Nutrition, National Engineering Laboratory of Intelligent Food Technology and Equipment, Key Laboratory for Agro-Products Postharvest Handling of Ministry of Agriculture, Zhejiang Key Laboratory for Agro-Food Processing, Fuli Institute of Food Science, Zhejiang University, Hangzhou 310058, China.
Laboratory of Fruit Quality Biology, Zhejiang Provincial Key Laboratory of Horticultural Plant Integrative Biology, The State Agriculture Ministry Laboratory of Horticultural Plant Growth, Development and Quality Improvement, Zhejiang University, Hangzhou 310058, China.
Food Res Int. 2018 Jan;103:326-334. doi: 10.1016/j.foodres.2017.10.062. Epub 2017 Oct 31.
This study was aimed to investigate the impact of in vitro gastrointestinal digestion on some common and new bayberry cultivars. The contents of total phenolics (246-669mg gallic acid equivalents/kg FW (fresh weight)), flavonoids (116-689mg quercetin-3-O-rutinoside equivalents/kg FW), procyanidins (28-133mg catechin equivalents/kg FW) and anthocyanins (1-7mg cyaniding-3-O-glucoside equivalents/kg FW) were detected in digested cultivars. HPLC-TOF-MS analysis identified 17 phenolic compounds in digested sample. Among all digested cultivars, the new cultivars Anhaizaomei (ABTS, IC=2.95mg/mL; FRAP, 401.32mg vitamin C equivalents (VCE)/kg FW) and Yingsi (ABTS, IC=3.28mg/mL; FRAP, 400.81mg VCE/kg FW) showed better in vitro antioxidant capacity. Further cellular assay indicated that the common cultivar Dongkui (2mg/mL) possessed the strongest ROS scavenging activity. The comprehensive evaluation of bioactive components and antioxidant properties using principal component analysis suggests that common cultivar Dongkui, new cultivars Yingsi and Anhaizaomei could be considered as dietary supplements.
本研究旨在探讨体外胃肠道消化对一些常见和新型杨梅品种的影响。在消化后的品种中检测到总酚(246-669mg 没食子酸当量/kg FW(鲜重))、类黄酮(116-689mg 槲皮素-3-O-鼠李糖苷当量/kg FW)、原花青素(28-133mg 儿茶素当量/kg FW)和花色苷(1-7mg 氰化-3-O-葡萄糖苷当量/kg FW)的含量。HPLC-TOF-MS 分析鉴定了消化后样品中的 17 种酚类化合物。在所有消化后的品种中,新型品种安海早梅(ABTS,IC=2.95mg/mL;FRAP,401.32mg 维生素 C 当量(VCE)/kg FW)和映霜(ABTS,IC=3.28mg/mL;FRAP,400.81mg VCE/kg FW)表现出更好的体外抗氧化能力。进一步的细胞试验表明,常见品种东魁(2mg/mL)具有最强的 ROS 清除活性。使用主成分分析对生物活性成分和抗氧化性能进行综合评价表明,常见品种东魁、新型品种映霜和安海早梅可作为膳食补充剂。