Camargo Livia Roberta Piedade, de Carvalho Vania Maria, Díaz Ingrit Elida Collantes, Paciencia Mateus Luís Barradas, Frana Sergio Alexandre, Younes Riad Naim, Varella Antonio Drauzio, Reis Luiz Fernando Lima, Suffredini Ivana Barbosa
Graduate Program in Environmental and Experimental Pathology, Paulista University, São Paulo, Brazil.
Department of Chemistry Engineer, Chemistry and Textile Engineer Faculty, Engineer National University, Lima, Peru.
Vet World. 2020 Jul;13(7):1376-1387. doi: 10.14202/vetworld.2020.1376-1387. Epub 2020 Jul 21.
is one of the main pathogens responsible for veterinary and human infections, and it is associated with significant economic losses in the livestock, as it causes severe diseases to humans, particularly in children. For that reason, there is a need for introducing new drugs to treat diseases. The Brazilian species richness is a source of potential new antibacterial natural products. The study aimed at the biological and chemical investigation of the organic extract obtained from the stem of a (Apocynaceae), EB127, as it was identified as a potential source of new antibacterial compounds to be used in Veterinary.
The antibacterial activity was evaluated by disk diffusion and microdilution assays; chromatography, nuclear magnetic resonance spectrometry, and mass spectrometry were used in the isolation and identification of compounds.
EB127 showed activity against ATCC25922, and against three strains that were isolated from frigarte's cloaca, named 31/1A, 35A, and 51A. Lupeol, 3-acetyl-11-oxo-β-amyrin, 3-acetyl-11-oxo-α-amyrin, sitosterol, stigmasterol, 3β,7α-dihydroxy-cholest-5-ene, 3β-hydroxy-cholest-5-en-7-one, and 3β-hydroxy-cholest-5,22-dien-7-one were identified in fraction Hex/CHCl, while loganin, loganic acid, methylanomaline, and anomaline were all identified in EB127 and protocatechuic acid hexoside, ferulic acid, secoxyloganin, feruloylquinic acid, vanillic acid hexoside, protocatechuic acid-4-O-β-hexoside, and rosmarinic acid were tentatively identified in fraction 10%ACN/HO. 51A (virulent/non-resistant) showed sensitivity to the antibacterial action of fraction Hex/CHCl which contains alkaloids, triterpenes, and steroids, while 35A (resistant/non-virulent) were more susceptible to 10%ACN/HO, which contains iridoids as loganin and loganic acid, and glycosylated and non-glycosylated caffeic acids.
Fraction 10%ACN/HO is of interest in pursuing new drugs to treat resistant , in veterinary. All compounds were isolated from the plant for the first time and have shown potential as new antibacterial natural products from Amazon plants to be used in veterinary and human diseases.
是引起兽医和人类感染的主要病原体之一，它给畜牧业造成重大经济损失，因为它会导致人类尤其是儿童患上严重疾病。因此，需要引入新药物来治疗疾病。巴西丰富的物种是潜在新型抗菌天然产物的来源。本研究旨在对从一种夹竹桃科植物的茎中获得的有机提取物EB127进行生物学和化学研究，因为它被确定为可用于兽医领域的新型抗菌化合物的潜在来源。
通过纸片扩散法和微量稀释法评估抗菌活性；使用色谱法、核磁共振光谱法和质谱法分离和鉴定化合物。
EB127对ATCC25922以及从弗氏泄殖腔分离出的三株菌株（命名为31/1A、35A和51A）具有活性。在己烷/氯仿馏分中鉴定出羽扇豆醇、3-乙酰基-11-氧代-β-香树脂醇、3-乙酰基-11-氧代-α-香树脂醇、豆甾醇、 stigmasterol、3β,7α-二羟基胆甾-5-烯、3β-羟基胆甾-5-烯-7-酮和3β-羟基胆甾-5,22-二烯-7-酮，而在EB127中鉴定出马钱子苷、马钱子酸、甲基异常山梗菜碱和异常山梗菜碱，在10%乙腈/水馏分中初步鉴定出原儿茶酸己糖苷、阿魏酸、裂环马钱子苷、阿魏酰奎尼酸、香草酸己糖苷、原儿茶酸-4-O-β-己糖苷和迷迭香酸。51A（有毒力/无抗性）对含有生物碱、三萜和甾体的己烷/氯仿馏分的抗菌作用敏感，而35A（有抗性/无毒力）对含有马钱子苷和马钱子酸等环烯醚萜以及糖基化和非糖基化咖啡酸的10%乙腈/水更敏感。
10%乙腈/水馏分在研发治疗兽医领域耐药性的新药物方面具有研究价值。所有化合物均首次从该植物中分离得到，并显示出作为亚马逊植物来源的新型抗菌天然产物用于兽医和人类疾病的潜力。
